Tabatabaei Vakili Saleh, Zeidi Roghayeh, Nabhani Robab
Department of Animal Science, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
Vet Med Sci. 2025 Mar;11(2):e70241. doi: 10.1002/vms3.70241.
Sperm membranes, rich in polyunsaturated fatty acids, are highly susceptible to lipid peroxidation. Oxidative stress significantly impacts sperm function, and B-group vitamins play a crucial role in mitigating this damage.
This study investigated the effects of vitamins B1 (VitB1) and B12 (VitB12) on sperm quality and seminal antioxidant status following cryopreservation in Arabi rams.
Semen was collected from 10 Arabi rams and pooled. The pooled semen was diluted and cryopreserved with varying concentrations of VitB1 and VitB12 (0, 0.5, 1, 2 and 4 mg/mL). Post-thaw sperm quality parameters, including motility, viability, membrane integrity and morphology, were assessed. In addition, seminal antioxidant enzyme activities and lipid peroxidation were evaluated.
Supplementation with 1 mg/mL VitB12 and 0.5-1 mg/mL VitB1 significantly enhanced post-thaw sperm motility, viability, membrane integrity and morphology. This was accompanied by a reduction in malondialdehyde (MDA) levels. The highest total antioxidant capacity (TAC) was observed with 1 mg/mL VitB12 and 0.5 mg/mL VitB1 (p < 0.05), positively correlating with sperm motility and viability (p < 0.01). Glutathione peroxidase (GPx) activity was highest in the 1 mg/mL VitB12 and VitB1 groups and negatively correlated with sperm abnormalities (p < 0.01). Catalase (CAT) activity significantly increased in the 1-2 mg/mL VitB12 and 0.5-2 mg/mL VitB1 groups, positively correlating with sperm progressive motility and viability (p < 0.01).
These findings demonstrate that incorporating 1 mg/mL VitB12 and 0.5 mg/mL VitB1 into the cryopreservation extender effectively mitigates oxidative stress, enhances antioxidant defences and improves post-thaw semen quality in Arabi rams.
富含多不饱和脂肪酸的精子膜极易发生脂质过氧化。氧化应激显著影响精子功能，而B族维生素在减轻这种损伤方面起着关键作用。
本研究调查了维生素B1（VitB1）和维生素B12（VitB12）对阿拉伯公羊冷冻保存后精子质量和精液抗氧化状态的影响。
从10只阿拉伯公羊采集精液并混合。将混合精液用不同浓度的VitB1和VitB12（0、0.5、1、2和4mg/mL）进行稀释和冷冻保存。评估解冻后精子质量参数，包括活力、存活率、膜完整性和形态。此外，还评估了精液抗氧化酶活性和脂质过氧化情况。
添加1mg/mL VitB12和0.5 - 1mg/mL VitB1可显著提高解冻后精子的活力、存活率、膜完整性和形态。同时丙二醛（MDA）水平降低。1mg/mL VitB12和0.5mg/mL VitB1组的总抗氧化能力（TAC）最高（p < 0.05），与精子活力和存活率呈正相关（p < 0.01）。1mg/mL VitB12和VitB1组的谷胱甘肽过氧化物酶（GPx）活性最高，与精子异常率呈负相关（p < 0.01）。1 - 2mg/mL VitB12和0.5 - 2mg/mL VitB1组的过氧化氢酶（CAT）活性显著增加，与精子前向运动率和存活率呈正相关（p < 0.01）。
这些研究结果表明，在冷冻保存稀释液中加入1mg/mL VitB12和0.5mg/mL VitB1可有效减轻氧化应激，增强抗氧化防御能力，并改善阿拉伯公羊解冻后精液质量。
