Department of Animal Science, Faculty of Agricultural and Engineering Science, Razi University, Kermanshah, Iran.
Department of Animal Science, National Institute of Genetics and Biotechnology, Tehran, Iran.
Vet Med Sci. 2024 Nov;10(6):e70091. doi: 10.1002/vms3.70091.
This study looked at how different concentrations of curcumin (Curc), zinc chloride (ZnCl), zinc oxide nanoparticles (ZnO-NPs) and Curc loaded on ZnO-NPs (Curc-co-ZnO-NPs) in cryopreservation dilution affected the quality of ram sperm after thawing.
ZnO-NPs were synthesised using Berberis vulgaris leaf aqueous extract. Then, Curc was loaded on the ZnO-NPs that had been synthesised. We used analytical methods to look at the composition, morphology and size of green synthesised ZnO-NPs and Curc-co-ZnO-NPs, including UV-Vis, zeta potential, EDX, DLS, FE-SEM and FT-IR. Using a Tris-base extender containing various concentrations of Curc, ZnCl, ZnO-NPs and Curc-co-ZnO-NPs (0, 1, 10 and 100 µg/mL), semen samples from four rams were combined. Sperm motility, viability, DNA and plasma membrane integrity, total abnormalities and malondialdehyde (MDA) generation were all evaluated in treatment groups after thawing.
The results showed that adding 1 µg/mL of ZnO-NPs and Curc-co-ZnO-NPs significantly reduced the level of MDA and total abnormalities (p < 0.05). Additionally, following the freeze-thawing procedure, the presence of 1 µg/mL of Curc-co-ZnO-NPs in the diluent of ram sperm significantly increased the percentage of sperm viability and motility in comparison to the control and other treatment groups (p < 0.05). Furthermore, as compared to the control group and other treatments, treatments containing 1 µg/mL of Curc-co-ZnO-NPs significantly improved membrane and DNA integrity (p < 0.05).
It appears that following freeze-thawing, the Curc-co-ZnO-NPs (1 µg/mL) enhanced sperm parameters.
本研究探讨了不同浓度的姜黄素（Curc）、氯化锌（ZnCl）、氧化锌纳米粒子（ZnO-NPs）和负载在 ZnO-NPs 上的姜黄素（Curc-co-ZnO-NPs）在冷冻保存稀释液中对解冻后公羊精子质量的影响。
使用小檗叶水提物合成 ZnO-NPs。然后，将姜黄素负载到已合成的 ZnO-NPs 上。我们使用包括紫外-可见分光光度法、zeta 电位、能谱、动态光散射、场发射扫描电子显微镜和傅里叶变换红外光谱在内的分析方法来研究绿色合成的 ZnO-NPs 和 Curc-co-ZnO-NPs 的组成、形貌和尺寸。使用含有不同浓度 Curc、ZnCl、ZnO-NPs 和 Curc-co-ZnO-NPs（0、1、10 和 100μg/mL）的 Tris 基础 extender 将来自四只公羊的精液样本混合。在解冻后评估处理组中精子的活力、活率、DNA 和质膜完整性、总畸形率和丙二醛（MDA）的产生。
结果表明，添加 1μg/mL 的 ZnO-NPs 和 Curc-co-ZnO-NPs 可显著降低 MDA 和总畸形率（p<0.05）。此外，在冻融处理后，与对照组和其他处理组相比，稀释液中含有 1μg/mL 的 Curc-co-ZnO-NPs 可显著提高精子活率和活力的百分比（p<0.05）。此外，与对照组和其他处理组相比，含有 1μg/mL 的 Curc-co-ZnO-NPs 的处理组可显著提高膜和 DNA 的完整性（p<0.05）。
似乎在冻融后，Curc-co-ZnO-NPs（1μg/mL）增强了精子参数。
