McDonald J K, Lumpkin M D, Samson W K, McCann S M
Peptides. 1985 Jan-Feb;6(1):79-84. doi: 10.1016/0196-9781(85)90080-4.
We have examined the effects of third cerebroventricular (3V) injections of avian and bovine pancreatic polypeptide (APP and BPP) and the C-terminal hexapeptide amide of human PP (CHPP) on the secretion of anterior pituitary hormones in conscious ovariectomized rats. Injection of APP (2.0 micrograms; 472 pmoles) or BPP (5.0 micrograms; 1191 pmoles) decreased plasma levels of luteinizing hormone (LH) when compared to pre-injection levels in these animals or to saline-injected controls. The lower dose of BPP (0.5 micrograms; 119 pmoles) decreased plasma LH versus pre-injection levels and control animals, however, these effects diminished at later times. Plasma growth hormone (GH) also decreased following 3V injections of APP (2.0 micrograms) or BPP (5.0 micrograms). The lower dose of BPP (0.5 microgram) initially inhibited GH release, however, this effect was rapidly reversed and GH levels were significantly greater than those in controls at 60 and 120 min. Injections of BPP or APP did not alter prolactin (PRL) or thyroid stimulating hormone (TSH) secretion. Administration of 2.0 micrograms and 0.2 microgram of CHPP (2488 and 249 pmoles) produced no significant effects on plasma LH, GH, PRL or TSH. APP and BPP had no consistent effects on hormone secretion from dispersed anterior pituitary cells. The results indicate that APP and BPP exert potent central effects which inhibit LH and GH release from the pituitary gland.
我们已经研究了向清醒的去卵巢大鼠第三脑室（3V）注射禽和牛胰多肽（APP和BPP）以及人PP的C末端六肽酰胺（CHPP）对垂体前叶激素分泌的影响。与这些动物的注射前水平或注射生理盐水的对照组相比，注射APP（2.0微克；472皮摩尔）或BPP（5.0微克；1191皮摩尔）可降低血浆促黄体生成素（LH）水平。较低剂量的BPP（0.5微克；119皮摩尔）与注射前水平和对照动物相比可降低血浆LH水平，然而，这些作用在随后的时间减弱。3V注射APP（2.0微克）或BPP（5.0微克）后血浆生长激素（GH）也降低。较低剂量的BPP（0.5微克）最初抑制GH释放，然而，这种作用迅速逆转，在60和120分钟时GH水平显著高于对照组。注射BPP或APP不会改变催乳素（PRL）或促甲状腺激素（TSH）的分泌。给予2.0微克和0.2微克的CHPP（2488和249皮摩尔）对血浆LH、GH、PRL或TSH没有显著影响。APP和BPP对分散的垂体前叶细胞的激素分泌没有一致的影响。结果表明，APP和BPP发挥强大的中枢作用，抑制垂体释放LH和GH。
