Huang X Y, McCann S M
Am J Physiol. 1983 Nov;245(5 Pt 1):E502-7. doi: 10.1152/ajpendo.1983.245.5.E502.
The effects of third ventricular injection of naloxone and atropine, alone and together, on plasma concentrations of luteinizing hormone (LH), follicle-stimulating hormone (FSH), prolactin (PRL), growth hormone (GH), and thyroid-stimulating hormone (TSH) were studied in conscious, ovariectomized (OVX) rats. Samples were removed from unrestrained rats that had been previously implanted with an atrial cannula, and plasma hormone levels were determined by radioimmunoassay. Intraventricular injection of naloxone (30 micrograms) induced increases in plasma LH and GH concentrations, decreased plasma PRL levels, but did not alter plasma TSH. When atropine (100 micrograms) was injected intraventricularly, there was a decrease in plasma LH and an increase in PRL levels but no alterations in the levels of the other hormones. The action of naloxone on PRL and GH release was eliminated by simultaneous administration of atropine, but there was only a slight reduction in its effect on LH. The results indicate that central cholinergic neurons may play an important role, via muscarinic type receptors, in mediation of the effects of naloxone and presumably opiates on PRL and GH release, whereas the effects on LH appear to proceed independently of cholinergic neurons.
在清醒的去卵巢（OVX）大鼠中，研究了单独及联合向第三脑室注射纳洛酮和阿托品对黄体生成素（LH）、促卵泡激素（FSH）、催乳素（PRL）、生长激素（GH）和促甲状腺激素（TSH）血浆浓度的影响。从先前已植入心房插管的未受约束的大鼠中采集样本，并用放射免疫分析法测定血浆激素水平。脑室内注射纳洛酮（30微克）可导致血浆LH和GH浓度升高，血浆PRL水平降低，但不改变血浆TSH水平。当向脑室内注射阿托品（100微克）时，血浆LH降低，PRL水平升高，但其他激素水平无变化。同时给予阿托品可消除纳洛酮对PRL和GH释放的作用，但对其对LH的作用仅有轻微降低。结果表明，中枢胆碱能神经元可能通过毒蕈碱型受体在介导纳洛酮以及可能的阿片类药物对PRL和GH释放的作用中发挥重要作用，而对LH的作用似乎独立于胆碱能神经元进行。
