紫外线照射后黄曲霉毒素诱变活性的降低。

Reduction of mutagenic activity of aflatoxins after UV-irradiation.

作者信息

Kleinwächter V, Koukalová B

出版信息

Acta Biol Med Ger. 1979;38(9):1239-42.

Abstract

Solutions of aflatoxins B1, B2, G1 and G2 and solid films of aflatoxin B1 were irradiated with UV light of wavelength corresponding to the absorption band of aflatoxins at approximately 365 nm (approximately 27 500 cm-1). The reaction was followed spectrophotometrically. Simultaneously the mutagenicity of samples of aflatoxins was determined using Salmonella typhimurium/mammalian microsome test. UV-irradiation caused a modification in all aflatoxins studied (in aqueous and nonaqueous solutions as well as in a solid film); the final photoproducts lost completely the mutagenic activity.

摘要

用波长约为365nm（约27500cm-1）、对应黄曲霉毒素吸收带的紫外光照射黄曲霉毒素B1、B2、G1和G2的溶液以及黄曲霉毒素B1的固体薄膜。通过分光光度法跟踪反应。同时，使用鼠伤寒沙门氏菌/哺乳动物微粒体试验测定黄曲霉毒素样品的诱变性。紫外线照射使所有研究的黄曲霉毒素（在水溶液和非水溶液以及固体薄膜中）发生了变化；最终的光产物完全丧失了诱变活性。