光活化黄曲霉毒素对鼠伤寒沙门氏菌具有致突变性，并在体外与DNA共价结合。

Photoactivated aflatoxins are mutagens to Salmonella typhimurium and bind covalently to DNA in vitro.

作者信息

Israel-Kalinsky H, Tuch J, Roitelman J, Stark A A

出版信息

Carcinogenesis. 1982;3(4):423-9. doi: 10.1093/carcin/3.4.423.

DOI:10.1093/carcin/3.4.423

Abstract

The mutagenesis of Salmonella typhimurium TA100 and covalent binding in vitro of photoactivated aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1) and aflatoxin G2 (AFG2) were investigated. Covalent binding levels of 1140, 225, 330 and 8 pmol aflatoxin per mumol nucleotide phosphate were obtained for AFB1, AFG1, AFB2 and AFG2, respectively, at 50 microM mycotoxin after 2 h of irradiation. Mutant frequencies to histidine prototrophy wre 97, 19, 49 and 0 x 10(-6) for AFB1, AFG1, AFB2 and AFG2 respectively, after 2 h irradiation at 100 microM mycotoxin in the surviving fraction of the mutagenized cultures. Toxicity to Salmonella was 0.59, 0.03, 0.31 and 0 lethal hits under the conditions specified for mutagenesis for AFB1, AFG1, AFB2 and AFG2, respectively.

摘要

研究了鼠伤寒沙门氏菌TA100的诱变作用以及光活化黄曲霉毒素B1（AFB1）、黄曲霉毒素B2（AFB2）、黄曲霉毒素G1（AFG1）和黄曲霉毒素G2（AFG2）的体外共价结合。照射2小时后，在50微摩尔霉菌毒素存在的情况下，AFB1、AFG1、AFB2和AFG2每微摩尔核苷酸磷酸的共价结合水平分别为1140、225、330和8皮摩尔黄曲霉毒素。在诱变培养物的存活部分中，在100微摩尔霉菌毒素存在下照射2小时后，AFB1、AFG1、AFB2和AFG2对组氨酸原养型的突变频率分别为97、19、49和0×10⁻⁶。在规定的诱变条件下，AFB1、AFG1、AFB2和AFG2对沙门氏菌的毒性分别为0.59、0.03、0.31和0致死剂量。