Ellwood K C, Michaelis O E, Emberland J J, Bhathena S J
Proc Soc Exp Biol Med. 1985 Jun;179(2):163-7. doi: 10.3181/00379727-179-42079.
Genetically obese normotensive rats, LA/N-corpulent (cp), were fed ad libitum diets containing either 54% sucrose or cooked corn starch for 12 weeks. Twenty-four rats were used for the study; half were corpulent (cp/cp) and half were lean (cp/+ or +/+). Fasting levels of plasma insulin, glucose, corticosterone, glucagon and growth hormone, and activities of liver and epididymal fat pad glucose-6-phosphate dehydrogenase (G6PD), malic enzyme (ME), and liver and kidney glucose-6-phosphatase (G6Pase), fructose 1,6-diphosphatase (FDPase), and phosphoenolpyruvate carboxykinase (PEPCK) were measured. A significant phenotype effect was observed in insulin, corticosterone, growth hormone, and liver G6PD, ME, FDPase, and kidney PEPCK, G6Pase, FDPase, and epididymal fat pad G6PD and ME (corpulent greater than lean), and glucagon (lean greater than corpulent). Diet effect (sucrose greater than starch) was significant for plasma glucose, liver ME, and kidney G6Pase. Although not significant at the P less than 0.05 level, insulin, corticosterone, liver G6PD and FDPase and kidney FDPase tended to be higher in sucrose-fed rats. This study suggests that the corpulent rat is more lipogenic and gluconeogenic than the lean, and that the hormones responsible are effective in keeping both the lipogenic and gluconeogenic enzyme activity elevated.
遗传性肥胖正常血压大鼠，LA/N-肥胖（cp），随意喂食含54%蔗糖或熟玉米淀粉的日粮12周。24只大鼠用于该研究；一半为肥胖（cp/cp），一半为瘦型（cp/+或+/+）。测量空腹血浆胰岛素、葡萄糖、皮质酮、胰高血糖素和生长激素水平，以及肝脏和附睾脂肪垫葡萄糖-6-磷酸脱氢酶（G6PD）、苹果酸酶（ME），肝脏和肾脏葡萄糖-6-磷酸酶（G6Pase）、果糖1,6-二磷酸酶（FDPase）和磷酸烯醇式丙酮酸羧激酶（PEPCK）的活性。在胰岛素、皮质酮、生长激素、肝脏G6PD、ME、FDPase、肾脏PEPCK、G6Pase、FDPase以及附睾脂肪垫G6PD和ME（肥胖大鼠高于瘦型大鼠）和胰高血糖素（瘦型大鼠高于肥胖大鼠）方面观察到显著的表型效应。日粮效应（蔗糖高于淀粉）对血浆葡萄糖、肝脏ME和肾脏G6Pase有显著影响。尽管在P<0.05水平不显著，但喂食蔗糖的大鼠胰岛素、皮质酮、肝脏G6PD和FDPase以及肾脏FDPase往往较高。本研究表明，肥胖大鼠比瘦型大鼠更具脂肪生成和糖异生能力，且负责的激素能有效维持脂肪生成和糖异生酶活性升高。
