Kinoshita Satoru Naganawa, Taki Kyomi, Okamoto Fumika, Nomoto Mika, Takahashi Koji, Hayashi Yuki, Ohkanda Junko, Tada Yasuomi, Finkemeier Iris, Kinoshita Toshinori
Institute of Plant Biology and Biotechnology, University of Muenster, Muenster, Germany.
Graduate School of Science, Nagoya University, Nagoya, Japan.
Plant J. 2025 Feb;121(3):e70034. doi: 10.1111/tpj.70034.
Plant cell growth requires the elongation of cells mediated by cell wall remodelling and turgor pressure changes. The plasma membrane (PM) H-ATPase facilitates both cell wall loosening and turgor pressure changes by acidifying the apoplast of cells, referred to as acid growth. The acid growth theory is mostly established on the auxin-induced activation of PM H-ATPase in non-photosynthetic tissues. However, how PM H-ATPase affects the growth in photosynthetic tissues of Arabidopsis remains unclear. Here, a combination of transcriptomics and cis-regulatory element analysis was conducted to identify the impact of PM H-ATPase on global transcript levels and the molecular mechanism downstream of the PM H-ATPase. The PM H-ATPase activation increased transcript levels globally, especially cell wall modification-related genes. The transcript level changes were in PM H-ATPase-dependent manner. Involvement of Ca was suggested as CAMTA motif was enriched in the promoter of PM H-ATPase-induced genes and cytosolic Ca elevated upon PM H-ATPase activation. PM H-ATPase activation in photosynthetic tissues promotes the expression of cell wall modification enzymes and shoot growth, adding a novel perspective of photosynthesis-dependent PM H-ATPase activation in photosynthetic tissues to the acid growth theory that has primarily based on findings from non-photosynthetic tissues.
植物细胞生长需要通过细胞壁重塑和膨压变化介导的细胞伸长。质膜（PM）H⁺-ATP酶通过酸化细胞的质外体促进细胞壁松弛和膨压变化，这被称为酸生长。酸生长理论主要建立在非光合组织中生长素诱导的质膜H⁺-ATP酶激活的基础上。然而，质膜H⁺-ATP酶如何影响拟南芥光合组织中的生长仍不清楚。在这里，进行了转录组学和顺式调控元件分析的组合，以确定质膜H⁺-ATP酶对全局转录水平的影响以及质膜H⁺-ATP酶下游的分子机制。质膜H⁺-ATP酶激活使全局转录水平增加，尤其是与细胞壁修饰相关的基因。转录水平变化是以质膜H⁺-ATP酶依赖性方式进行的。由于CAMTA基序在质膜H⁺-ATP酶诱导基因的启动子中富集且质膜H⁺-ATP酶激活后胞质Ca²⁺升高，提示Ca²⁺参与其中。光合组织中质膜H⁺-ATP酶激活促进细胞壁修饰酶的表达和茎的生长，为酸生长理论增添了光合组织中依赖光合作用的质膜H⁺-ATP酶激活的新视角，该理论主要基于非光合组织的研究结果。
