Geddes Barney A, Williamson Riley, Schumacher Jake, Ardi Ahmad, Levin Garrett, Červenka Emily, Huang Rui, diCenzo George C
Department of Microbiological Sciences, North Dakota State University, Fargo, ND, USA.
Department of Biology, Queen's University, Kingston, ON, Canada.
Can J Microbiol. 2025 Jan 1;71:1-13. doi: 10.1139/cjm-2024-0246.
This expansion for the modular vector assembly platform BEVA (Bacterial Expression Vector Archive) introduces 11 new BEVA parts including two new cloning site variants, two new antibiotic resistance modules, three new origins of replication, and four new accessary modules. As a result, the modular system is now doubled in size and expanded in its capacity to produce diverse replicating plasmids. Furthermore, it is now amenable to genetic engineering methods involving genome-manipulation of target strains through deletions or integrations. In addition to introducing the new modules, we provide several BEVA-derived Golden Gate cloning plasmids that are used to validate parts and that may be useful for genetic engineering of proteobacteria and other bacteria. We also introduce new parts to allow compatibility with the CIDAR MoClo parts libraries.
模块化载体组装平台BEVA（细菌表达载体文库）的此次扩展引入了11个新的BEVA元件，包括两个新的克隆位点变体、两个新的抗生素抗性模块、三个新的复制起点和四个新的辅助模块。结果，该模块化系统的规模现在扩大了一倍，产生各种复制质粒的能力也得到了扩展。此外，它现在适用于通过缺失或整合对目标菌株进行基因组操作的基因工程方法。除了引入新模块外，我们还提供了几种源自BEVA的金门克隆质粒，用于验证元件，可能对变形菌和其他细菌的基因工程有用。我们还引入了新元件，以实现与CIDAR MoClo元件文库的兼容性。
