Goda Doaa A, El-Gamal Eman H, Rashad Mohamed, Abdel-Fattah Yasser R
Bioprocess Development Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), Universities and Research Institutes Zone, New Borg El-Arab City, Alexandria, 21934, Egypt.
Land and Water Technologies Department, Arid Lands Cultivation Research Institute (ALCRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Alexandria, 21934, Egypt.
Sci Rep. 2025 Feb 8;15(1):4676. doi: 10.1038/s41598-025-86941-9.
Adding organic amendments to agricultural systems as sustainable practices is one of the most important stages toward creating a more sustainable and environmentally friendly food system. By increasing soil fertility and nutritional content, organic fertilizers increase plant productivity. Cation Exchange Capacity (CEC) is a vital indicator of soil fertility and plant nutrient availability, which is considered one of the most significant chemical parameters that affect agricultural soil quality. The main goals of the present study were to generate protein lysate from discarded feathers (enriched in different amino acids) through solid-state fermentation using Box-Behnken design (BBD). Then, assess the efficacy of hydrolyzed feather product (as an organic amendment), time, and N-P fertilizers on soil-CEC planted with maize in calcareous soil utilizing an optimization strategy that employed the central composite design and the response surface methodology (RSM). The results revealed that the protein concentration in the customized conditions was 1173.53 µg ml. With a predicted CEC of 31.416 cmol kg, the ideal circumstances for the three variables under investigation of feather hydrolysate, time, and chemical fertilizer were 20.147 ml kg soil, 27 days, and 42.3% of the recommended dose, respectively. Using keratin hydrolysate (20 ml kg soil) as a soil amendment significantly improved the growth parameters of maize. The leaf surface area (SA, m gm) was increased by 695% and 37% compared to the control (without any addition) and N-P fertilizer treatments (100% of the recommended dose), respectively. Keratin hydrolysate as a sustainable production of value-added organic fertilizer applied to calcareous soil shows a synergistic effect on soil-CEC and plant growth parameters.
添加有机改良剂到农业系统作为可持续发展的做法，是迈向创建更可持续和环境友好型食品系统的最重要阶段之一。通过提高土壤肥力和营养成分，有机肥料可提高作物产量。阳离子交换容量（CEC）是土壤肥力和植物养分有效性的重要指标，被认为是影响农业土壤质量的最重要化学参数之一。本研究的主要目标是通过使用Box-Behnken设计（BBD）的固态发酵从废弃羽毛（富含不同氨基酸）中生成蛋白质裂解物。然后，利用采用中心复合设计和响应面方法（RSM）的优化策略，评估水解羽毛产品（作为有机改良剂）、时间和氮磷肥料对种植在石灰性土壤中的玉米土壤CEC的影响。结果表明，定制条件下的蛋白质浓度为1173.53 µg/ml。对于羽毛水解物、时间和化肥这三个研究变量，预测CEC为31.416 cmol/kg时的理想条件分别是20.147 ml/kg土壤、27天和推荐剂量的42.3%。使用角蛋白水解物（20 ml/kg土壤）作为土壤改良剂可显著改善玉米的生长参数。与对照（不添加任何物质）和氮磷肥料处理（推荐剂量的100%）相比，叶片表面积（SA，m²/g）分别增加了695%和37%。角蛋白水解物作为一种应用于石灰性土壤的增值有机肥料的可持续生产方式，对土壤CEC和植物生长参数具有协同效应。
