Akhlynina T V, Buzhurina I M, Panov M A, Rozovskaia I A, Chernaia N G
Tsitologiia. 1985 Mar;27(3):348-52.
Ribosomal RNA (rRNA) synthesis in the intact Ehrlich ascite carcinoma cells is selectively inhibited by papaverin (ED50 = 0.01 mM), 2,4-dinitrophenol (DPN; ED50 = 5 microM), and actinomycin D (ED50 = 0.1 microgram/ml). The inhibition of rRNA synthesis is not connected with a direct action of these agents on the rRNA synthesis apparatus, and they had no effect on isolated cell nuclei. The rRNA synthesis in cells permeabilized with triton X-100 (0.05%) becomes insensible to the action of papaverine and DPN, but is inhibited by actinomycin D in low doses. In cells permeabilized with digitonin (0.01%) the rRNA synthesis shows no sensibility to the action of low doses of actinomycin D. The results suggest that the action of these agents on the rRNA synthesis may depend on cell integrity and on the permeabilization method employed.
完整的艾氏腹水癌细胞中的核糖体RNA（rRNA）合成受到罂粟碱（ED50 = 0.01 mM）、2,4-二硝基苯酚（DPN；ED50 = 5 microM）和放线菌素D（ED50 = 0.1微克/毫升）的选择性抑制。rRNA合成的抑制与这些药物对rRNA合成装置的直接作用无关，并且它们对分离的细胞核没有影响。用曲拉通X-100（0.05%）通透的细胞中的rRNA合成对罂粟碱和DPN的作用变得不敏感，但低剂量的放线菌素D可抑制其合成。在用洋地黄皂苷（0.01%）通透的细胞中，rRNA合成对低剂量放线菌素D的作用不敏感。结果表明，这些药物对rRNA合成的作用可能取决于细胞完整性和所采用的通透方法。
