da Costa Silva Jayara Dayany, Silva Paulo Roberto Ramalho, de França Solange Maria, Saleh Ibrahim A, de Alcântara Neto Francisco, El-Tayeb Mohamed A, Matos Kedma da Silva, Abdel-Maksoud Mostafa A, Guimarães Sarah da Silva Costa, Zuffo Alan Mario, da Silva Gilvan Ferreira, de Farias Antônio Roberto Gomes, Briozo Marcus Eugênio Oliveira, AbdElgawad Hamada, de Melo Maruzanete Pereira
Department of Agronomy, Federal Institute of Piauí, Oeiras, Piauí, Brazil.
Department of Plant Science, Federal University of Piauí, Teresina, Piauí, Brazil.
PLoS One. 2025 Feb 10;20(2):e0310548. doi: 10.1371/journal.pone.0310548. eCollection 2025.
The Metarhizium anisopliae complex consists of 34 formally described phylogenetic species. Metarhizium anisopliae sensu lato has been used for decades in Brazil as a biological control agent for controlling spittlebugs in sugarcane plantations. We investigated the identities of the Metarhizium isolates used in mycoinsecticide formulations through multilocus phylogenetic analyses and morphological characterization for species delimitation. A well-supported clade containing only isolates from this study formed a sister group with species of M. anisopliae sensu stricto, which we described as a new taxon, M. mendonceae sp. nov. Isolates URM 8144 and URM 8145 are used in the formulation of various brands of biological insecticides; however, they have always been referred to as M. anisopliae. According to the antibiosis assay, all the isolates of this new species were able to colonize and kill Mahanarva spectabilis nymphs. Therefore, M. mendonceae has been used in the formulation of mycoinsecticides for several decades under the name M. anisopliae.
绿僵菌复合体由34个正式描述的系统发育种组成。在巴西，广义绿僵菌已被用作生物防治剂数十年，用于控制甘蔗种植园中的沫蝉。我们通过多位点系统发育分析和形态特征鉴定来确定用于杀真菌剂配方中的绿僵菌分离株的身份，以进行物种划分。一个仅包含本研究分离株的得到充分支持的分支，与狭义绿僵菌的物种形成了姐妹群，我们将其描述为一个新分类单元，即门东塞绿僵菌（Metarhizium mendonceae sp. nov.）。分离株URM 8144和URM 8145被用于多种品牌生物杀虫剂的配方中；然而，它们一直被称为绿僵菌。根据抗菌试验，该新物种的所有分离株都能够定殖并杀死壮观大褐飞虱若虫。因此，门东塞绿僵菌以绿僵菌的名义被用于杀真菌剂配方已有数十年。
