Gilmore Annika L, Vu Helena, Martinez Travis, Peniata Lousili, Kawaguchi Brooke, Armbruster David A, Ashton Nicholas N, Williams Dustin L
Department of Biomedical Engineering, University of Utah, Salt Lake City, Utah, United States of America.
Department of Orthopaedics, University of Utah, Salt Lake City, Utah, United States of America.
PLoS One. 2025 Feb 10;20(2):e0318487. doi: 10.1371/journal.pone.0318487. eCollection 2025.
Laboratory grown biofilms are used to simulate bacterial growth in diverse environmental conditions and screen the effectiveness of anti-biofilm therapies. Recently, we developed a glass bead biofilm reactor that utilizes low broth volume to provide high-throughput biofilm growth for testing and translation across the research continuum (e.g., benchtop assays to preclinical models). Bioburden per mm2 surface area of Staphylococcus aureus and Pseudomonas aeruginosa biofilms were comparable on beads and CDC Biofilm Reactor® coupons. In this study, we hypothesized that biofilms grown on beads would be more susceptible to ertapenem, moxifloxacin, and tobramycin than those grown on coupons. Results indicated a significant reduction in S. aureus bioburden on glass beads compared to glass coupons following treatment with ertapenem (p = 0.005) and tobramycin (p = 0.014). P. aeruginosa biofilms had smaller differences in antibiotic response between the two systems. There was a significantly greater reduction in bead P. aeruginosa biofilm than coupon when treated with tobramycin (p = 0.035). This work offered insight into how the bead biofilm reactor could be used as a tool for antibiotic screening and translation across the continuum of in vitro to in vivo systems that support development of antimicrobial technology.
实验室培养的生物膜用于模拟细菌在不同环境条件下的生长，并筛选抗生物膜疗法的有效性。最近，我们开发了一种玻璃珠生物膜反应器，它利用少量肉汤来提供高通量生物膜生长，以便在整个研究过程中进行测试和转化（例如，从台式试验到临床前模型）。金黄色葡萄球菌和铜绿假单胞菌生物膜每平方毫米表面积的生物负荷在珠子和疾控中心生物膜反应器®试片上相当。在本研究中，我们假设在珠子上生长的生物膜比在试片上生长的生物膜对厄他培南、莫西沙星和妥布霉素更敏感。结果表明，用厄他培南（p = 0.005）和妥布霉素（p = 0.014）处理后，玻璃珠上金黄色葡萄球菌的生物负荷与玻璃试片相比显著降低。铜绿假单胞菌生物膜在这两种系统中的抗生素反应差异较小。用妥布霉素处理时，珠子上的铜绿假单胞菌生物膜比试片上的生物膜减少得更显著（p = 0.035）。这项工作为玻璃珠生物膜反应器如何用作抗生素筛选工具以及在支持抗菌技术开发的体外到体内系统连续过程中的转化提供了见解。
