Lin Yabin, Guo Qixuan, Liu Qing, Wang Weikang, Lv Ang, Zhang Luping, Li Liming, Gao Jianqing, Huang Fei
Institute of Neurobiology, Binzhou Medical University, 346 Guanhai Road, Laishan Yantai 264003, Shandong, China.
Department of Anatomy, School of Basic Medicine, Shandong University, Jinan, Shandong 250021, China; School of Health and Life Sciences, University of Health and Rehabilitation Sciences, Qingdao 266071, China.
Int J Biol Macromol. 2025 Apr;304(Pt 1):140819. doi: 10.1016/j.ijbiomac.2025.140819. Epub 2025 Feb 8.
In the early stage of spinal cord injury (SCI), excessive reactive oxygen species (ROS) accumulate at the injury site to establish a microenvironment favoring complex secondary injuries, including cavity formation, and impacting the distal lumbosacral spinal cord. Currently, no definitive clinical treatment is available for SCI. Here, we created an responsive and injectable hydrogel composite (GEL-EXO) by modifying and cross-linking biological macromolecules sodium alginate (SA) and gelatin in the form of embedded exosomes. This GEL-EXO composite integrated the bioactivity of exosomes with the gap-filling function of hydrogels. Our experiments demonstrated that the composite could simultaneously repair spinal cord tissues at the injury site and the distal lumbosacral region, thereby restoring motor function and reinitiating bladder function. This therapeutic strategy may promise a brand-new holistic intervention for SCI.
在脊髓损伤（SCI）的早期阶段，过量的活性氧（ROS）在损伤部位积累，从而形成一个有利于包括空洞形成在内的复杂继发性损伤的微环境，并影响腰骶部远端脊髓。目前，尚无明确的SCI临床治疗方法。在此，我们通过修饰生物大分子海藻酸钠（SA）和明胶并以包埋外泌体的形式使其交联，制备了一种可响应且可注射的水凝胶复合材料（GEL-EXO）。这种GEL-EXO复合材料将外泌体的生物活性与水凝胶的填充间隙功能结合在一起。我们的实验表明，该复合材料能够同时修复损伤部位和腰骶部远端区域的脊髓组织，从而恢复运动功能并重新启动膀胱功能。这种治疗策略可能为SCI带来一种全新的整体干预方法。
