Wu Meng-Yuan, Peng Cheng, Meng Chun-Wang, Liu Juan-Ru, Zhou Qin-Mei, Dai Ou, Xiong Liang
State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy/School of Modern Chinese Medicine Industry, Chengdu University of Traditional Chinese Medicine Chengdu 611137, China.
State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy/School of Modern Chinese Medicine Industry, Chengdu University of Traditional Chinese Medicine Chengdu 611137, China College of Medical Technology, Chengdu University of Traditional Chinese Medicine Chengdu 611137, China.
Zhongguo Zhong Yao Za Zhi. 2025 Jan;50(1):152-171. doi: 10.19540/j.cnki.cjcmm.20241014.201.
This study established a high performance liquid chromatography(HPLC) fingerprint of Chuanxiong Rhizoma from different producing areas and screened its potential differential components for producing areas by chemometrics. Furthermore, the content of the above differential components in Chuanxiong Rhizoma from different producing areas was measured and compared. Then, the geoherbalism markers(geo-markers) that can be used to distinguish Dao-di and non-Dao-di Chuanxiong Rhizoma were excavated by chemometrics. In fingerprint studies, a total of 27 common peaks were determined, and the fingerprint similarity for 37 batches of Chuanxiong Rhizoma samples from different producing areas was above 0.968. The orthogonal partial least squares-discriminant analysis(OPLS-DA) was capable of distinguishing Chuanxiong Rhizoma from Sichuan and from three other provinces, as well as Dao-di Chuanxiong Rhizoma(from Dujiangyan) and non-Dao-di Chuanxiong Rhizoma(from other producing areas) in Sichuan province. Meanwhile, 14 potential differential components in Chuanxiong Rhizoma from different provinces and 16 potential differential components in Chuanxiong Rhizoma from different producing areas in Sichuan were screened by the variable importance in projection(VIP) analysis under OPLS-DA. The reference standards were used to identify 10 potential differential components in the common peaks, and subsequent content determination verified that the content of the above 10 potential differential components was different among different producing areas. Then, the OPLS-DA and VIP analysis were performed with the content of the 10 potential differential components as variables. The results showed that Z-ligustilide, chlorogenic acid, and the ratio of butylidenephthalide/senkyunolide A were the geo-markers that can distinguish Chuanxiong Rhizoma from Sichuan and Chuanxiong Rhizoma from Shaanxi, Hebei, and Jiangxi, while Z-ligustilide, n-butylphthalide, and the ratios of Z-ligustilide/senkyunolide A and butylidenephthalide/senkyunolide A were the geo-markers that can distinguish Dao-di Chuanxiong Rhizoma(from Dujiangyan) and non-Dao-di Chuanxiong Rhizoma(from other producing areas) in Sichuan province. This study elucidated the differences in material basis of Dao-di and non-Dao-di Chuanxiong Rhizoma based on fingerprinting and content determination combined with chemometrics, which provides a reference for the study of material basis of Dao-di traditional Chinese medicine.
本研究建立了不同产地川芎的高效液相色谱（HPLC）指纹图谱，并通过化学计量学筛选其产地潜在差异成分。此外，测定并比较了不同产地川芎中上述差异成分的含量。然后，通过化学计量学挖掘出可用于区分道地与非道地产区川芎的道地性标志物（geo-markers）。在指纹图谱研究中，共确定了27个共有峰，37批次不同产地川芎样品的指纹图谱相似度均在0.968以上。正交偏最小二乘判别分析（OPLS-DA）能够区分四川川芎与其他三省川芎，以及四川省内道地川芎（都江堰产）与非道地川芎（其他产地）。同时，通过OPLS-DA下的变量投影重要性（VIP）分析，筛选出不同省份川芎中的14个潜在差异成分以及四川省不同产地川芎中的16个潜在差异成分。采用对照品对共有峰中的10个潜在差异成分进行鉴定，后续含量测定证实上述10个潜在差异成分在不同产地间含量存在差异。然后，以这10个潜在差异成分的含量为变量进行OPLS-DA和VIP分析。结果表明，Z-藁本内酯、绿原酸以及丁烯基苯酞/藁本内酯A的比值是区分四川川芎与陕西、河北、江西川芎的道地性标志物，而Z-藁本内酯、正丁基苯酞以及Z-藁本内酯/藁本内酯A和丁烯基苯酞/藁本内酯A的比值是区分四川省内道地川芎（都江堰产）与非道地川芎（其他产地）的道地性标志物。本研究基于指纹图谱和含量测定并结合化学计量学阐明了道地与非道地川芎物质基础的差异，为道地中药材物质基础研究提供了参考。
