Bein Bernhard, Chrysostomakis Ioannis, Arantes Larissa S, Brown Tom, Gerheim Charlotte, Schell Tilman, Schneider Clément, Leushkin Evgeny, Chen Zeyuan, Sigwart Julia, Gonzalez Vanessa, Wong Nur Leena W S, Santos Fabricio R, Blom Mozes P K, Mayer Frieder, Mazzoni Camila J, Böhne Astrid, Winkler Sylke, Greve Carola, Hiller Michael
LOEWE Centre for Translational Biodiversity Genomics, Senckenberganlage 25, Frankfurt, 60325, Germany.
Senckenberg Research Institute, Senckenberganlage 25, Frankfurt, 60325, Germany.
Genome Biol. 2025 Feb 10;26(1):25. doi: 10.1186/s13059-025-03487-9.
Museum collections harbor millions of samples, largely unutilized for long-read sequencing. Here, we use ethanol-preserved samples containing kilobase-sized DNA to show that amplification-free protocols can yield contiguous genome assemblies. Additionally, using a modified amplification-based protocol, employing an alternative polymerase to overcome PCR bias, we assemble the 3.1 Gb maned sloth genome, surpassing the previous 500 Mb protocol size limit. Our protocol also improves assemblies of other difficult-to-sequence molluscs and arthropods, including millimeter-sized organisms. By highlighting collections as valuable sample resources and facilitating genome assembly of tiny and challenging organisms, our study advances efforts to obtain reference genomes of all eukaryotes.
博物馆馆藏保存着数以百万计的样本，这些样本在很大程度上未被用于长读长测序。在这里，我们使用含有千碱基大小DNA的乙醇保存样本，以表明无扩增方案可以产生连续的基因组组装。此外，我们使用一种改良的基于扩增的方案，采用替代聚合酶来克服PCR偏差，成功组装了31亿碱基对的鬃毛树懒基因组，超过了之前5亿碱基对的方案大小限制。我们的方案还改进了其他难以测序的软体动物和节肢动物的组装，包括毫米大小的生物。通过强调馆藏作为宝贵的样本资源，并促进微小且具有挑战性的生物的基因组组装，我们的研究推动了获取所有真核生物参考基因组的工作。
