通过聚合 SER 的数量性状基因座提高细胞质雄性不育系的异交能力。
Improvement of the outcrossing ability of CMS lines by pyramiding QTLs for SER.
作者信息
Tan Quanya, Gan Zhenpeng, Shao Lin, Xiong Liang, Luan Xin, Yang Weifeng, Zhu Haitao, Bu Suhong, Liu Guifu, Liu Zupei, Wang Shaokui, Zhang Guiquan
机构信息
Guangdong Provincial Key Laboratory of Plant Molecular Breeding, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642 China.
Key Laboratory of Biology and Germplasm Innovation of Perennial Rice From Ministry of Agriculture and Rural Affairs, School of Agriculture, Yunnan University, Kunming, 650500 China.
出版信息
Mol Breed. 2025 Feb 8;45(2):24. doi: 10.1007/s11032-025-01545-5. eCollection 2025 Feb.
UNLABELLED
The stigma exsertion rate (SER) is a key factor in improving the outcrossing ability of cytoplasmic male sterility (CMS) lines in rice. In previous studies, we identified 18 SER-QTLs and developed some SER-QTL pyramiding lines (PLs). In this study, 4QL-1 and 4QL-2 were selected from these PLs and crossed with CMS maintainer lines H211B and H212B, respectively, to develop two new CMS maintainer lines, H221B and H222B, and their CMS lines H221A and H222A. The SER of H221B and H222B were 74.7% and 73.1%, respectively, reaching a high SER level. Compared with CMS maintainer lines, the CMS lines consistently exhibited higher SER, which may be related to the delayed flowering time of the CMS lines. Filed experiments showed that outcrossing seed-setting rates of H221A and H222A were significantly higher than those of the original CMS lines, which meets the requirements for hybrid rice seed production. These results confirm that SER is a key factor in enhancing rice outcrossing ability. Our findings demonstrate that pyramiding SER-QTLs is an effective strategy for improving rice SER and increasing outcrossing seed-setting rate.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s11032-025-01545-5.
未标注
柱头外露率（SER）是提高水稻细胞质雄性不育（CMS）系异交能力的关键因素。在先前的研究中，我们鉴定出18个SER-QTL，并培育了一些SER-QTL聚合系（PL）。在本研究中，从这些PL中选出4QL-1和4QL-2，分别与CMS保持系H211B和H212B杂交，培育出两个新的CMS保持系H221B和H222B，以及它们对应的CMS系H221A和H222A。H221B和H222B的SER分别为74.7%和73.1%，达到了较高的SER水平。与CMS保持系相比，CMS系的SER始终较高，这可能与CMS系开花时间延迟有关。田间试验表明，H221A和H222A的异交结实率显著高于原始CMS系，符合杂交水稻制种要求。这些结果证实，SER是提高水稻异交能力的关键因素。我们的研究结果表明，聚合SER-QTL是提高水稻SER和增加异交结实率的有效策略。
补充信息
在线版本包含可在10.1007/s11032-025-01545-5获取的补充材料。
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