Kőrösi Dénes, Göcző Ágoston, Varga Noémi, Garamvölgyi Rita, Balogh Nándor, Farkas Kornélia, Vorobcsuk András
Doctoral School in Animal Science, Hungarian University of Agriculture and Life Sciences, Kaposvár, Hungary.
Medical School, University of Pécs, Pécs, Hungary.
Front Vet Sci. 2025 Jan 27;12:1493660. doi: 10.3389/fvets.2025.1493660. eCollection 2025.
The present study aimed to assess changes in biochemical parameters during the adaptation of the myocardial infarction model to a conventional Hungarian minipig breed. According to our hypothesis, changes in the blood level of the necroenzymes are not only related to the interventional procedure but are also influenced by peri-procedural animal keeping and treatment conditions.
Closed chest acute myocardial infarction (AMI) was induced by balloon occlusion for 90 min in the left anterior descendent coronary artery (LAD) in 24 adult, female Pannon minipigs followed by reperfusion. Blood samples were taken before AMI, and immediately after the reperfusion, during the cardiac magnetic resonance imaging (cMRI) on days 3 and 30. Aspartate transaminase (AST), alanine aminotransferase (ALT), creatine kinase (CK), lactate dehydrogenase (LDH), and high-sensitivity troponin I were determined.
While the parameters measured at baseline remained within physiological ranges, a notable elevation was seen in comparison with the results observed on day 30. This phenomenon was evident in all the laboratory parameters tested, except hs-troponin. The results for AST, ALT, LDH, and CK were statistically significant ( = 0.011, = 0.001, = 0.013, and = 0.001, respectively). A statistically significant difference was observed between the baseline and 30-day AST/ALT ratio ( = 0.00514).
The elevated levels of necroenzymes observed at baseline are likely to be a consequence of the physical and social stress imposed by the study design on the minipigs during the 72-h period prior to intervention. It is essential to define the optimal timing of baseline blood tests to ensure the reliability of the biochemical profile in a large animal infarction model.
本研究旨在评估心肌梗死模型在适应匈牙利传统小型猪品种过程中生化参数的变化。根据我们的假设，坏死酶血液水平的变化不仅与介入程序有关，还受围手术期动物饲养和治疗条件的影响。
对24只成年雌性潘诺尼亚小型猪，通过球囊闭塞左前降支冠状动脉（LAD）90分钟诱导闭胸急性心肌梗死（AMI），随后进行再灌注。在AMI前、再灌注后即刻、第3天和第30天心脏磁共振成像（cMRI）期间采集血样。测定天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、肌酸激酶（CK）、乳酸脱氢酶（LDH）和高敏肌钙蛋白I。
虽然基线测量参数仍在生理范围内，但与第30天观察结果相比有显著升高。除高敏肌钙蛋白外，所有测试实验室参数均出现此现象。AST、ALT、LDH和CK结果具有统计学意义（分别为 = 0.011、 = 0.001、 = 0.013和 = 0.001）。基线与第30天AST/ALT比值之间观察到统计学显著差异（ = 0.00514）。
基线时观察到的坏死酶水平升高可能是研究设计在干预前72小时内给小型猪带来的身体和社会应激的结果。确定基线血液检测的最佳时间对于确保大型动物梗死模型中生化谱的可靠性至关重要。
