白细胞介素10基因单核苷酸变异rs1800896（A>G）、rs1800871（C>T）、rs1800872（C>A）及单倍型与巴西人群中新冠病毒疾病严重程度和转归的关联
Association of IL10 gene SNVs rs1800896 (A>G), rs1800871 (C>T), rs1800872 (C>A) and haplotypes with COVID-19 severity and outcome in the Brazilian population.
作者信息
de Oliveira Janaina Nicolau, Fernandes Caroline Yukari Motoori, de Godoy Sara Mataroli, Frantine-Silva Wilson, de Souza Cassela Pedro Luis Candido, Trigo Guilherme Lerner, Lozovoy Marcell Alysson Batisti, Tano Zuleica Naomi, Simão Andrea Name Colado, de Oliveira Karen Brajão
机构信息
Laboratory of Molecular Genetics and Immunology, Department of Immunology, Parasitology and General Pathology, Center of Biological Sciences, State University of Londrina, Pr 445 km 380 Celso Garcia Cid Highway 86.057-970 PR, Brazil.
Laboratory for Studies and Analysis of Polymorphisms, Department of Immunology, Parasitology and General Pathology, Center of Biological Sciences, State University of Londrina 86.057-970 PR, Brazil.
出版信息
Hum Immunol. 2025 Mar;86(2):111261. doi: 10.1016/j.humimm.2025.111261. Epub 2025 Feb 10.
BACKGROUND
Elevated concentrations of IL-10 have been detected in coronavirus disease (COVID-19) patients and are a possible disease severity marker. Single nucleotide variants (SNVs) and their haplotypes can be associated with differences in IL-10 levels and with viral disease susceptibility.
AIM
Evaluate the associations of SNVs and their haplotypes in Brazilian patients with COVID-19 severity and outcome.
METHODS
In this cross-sectional and case-control study, the patients were selected from the University Hospital of State University of Londrina (HU-UEL) (n = 367) and were subdivided into mild (n = 165), moderate (n = 72) and severe (n = 130) groups. The DNA samples of the participants were subjected to real-time PCR for the detection of rs1800896 (A>G), rs1800871 (C>T) and rs1800872 (C>A) genotypes. The haplotypes were inferred with PHASE v2.1.1.
RESULTS
The severe cases of COVID-19 were independently associated with the GG genotype (rs1800896) (P = 0.038, OR 2.522, 95 % CI 1.053-6.038) as well as with the GCC haplotype in homozygosity (P = 0.037, OR 2.767, 95 % CI 1.065-7.191).
CONCLUSION
These results showed that the GG genotype of rs1800896 or the GCC haplotype are associated with COVID-19 severity in Brazilian patients.
背景
在冠状病毒病（COVID-19）患者中检测到白细胞介素-10（IL-10）浓度升高，其可能是疾病严重程度的标志物。单核苷酸变异（SNV）及其单倍型可能与IL-10水平差异及病毒病易感性相关。
目的
评估巴西COVID-19患者中SNV及其单倍型与疾病严重程度和转归的关联。
方法
在这项横断面病例对照研究中，患者选自隆德里纳州立大学大学医院（HU-UEL）（n = 367），并分为轻症组（n = 165）、中症组（n = 72）和重症组（n = 130）。对参与者的DNA样本进行实时聚合酶链反应，以检测rs1800896（A>G）、rs1800871（C>T）和rs1800872（C>A）基因型。用PHASE v2.1.1软件推断单倍型。
结果
COVID-19重症病例与GG基因型（rs1800896）独立相关（P = 0.038，比值比2.522，95%可信区间1.053 - 6.038），也与纯合子GCC单倍型独立相关（P = 0.037，比值比2.767，95%可信区间1.065 - 7.191）。
结论
这些结果表明，rs1800896的GG基因型或GCC单倍型与巴西患者的COVID-19严重程度相关。
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