C Sivaraju, Joseph Joby
University of Hyderabad, Hyderabad, Telangana, India, 500046.
Cell Tissue Res. 2025 Apr;400(1):35-49. doi: 10.1007/s00441-025-03956-4. Epub 2025 Feb 12.
Hieroglyphus banian (H. banian) is a grasshopper species, endemic to South Asia. The optic lobe in Acrididae has been characterized to a great extent in orthoptera, predominantly using Locust species like Schistocerca gregaria, Schistocerca americana, and Locusta migratoria, which are closely related to each other. In this work, we characterize the anatomical features of the optic lobe and associated pathway in the grasshopper species H. banian using tract tracing, immunohistochemistry, and intracellular fills. All the areas of the visual pathways that have been reported in the other orthoptera species could be identified in H. banian. Visual pathways exhibited similar structure and connectivity as shown in immunohistochemistry and tract-tracing results supporting the conservation of these features across species within Acrididae. Two new structures in the posterior protocerebrum, PS1 and PS2 with prominent innervations from the optic lobe were identified. Novel structure PS1 is innervated from medulla via PS1T and, PS2 from aMe via PS2T, both new tracts we have identified.
印度稻蝗（Hieroglyphus banian，简称H. banian）是一种原产于南亚的蝗虫。在直翅目中，蝗科昆虫的视叶已在很大程度上得到了表征，主要使用的是相互密切相关的物种，如沙漠蝗（Schistocerca gregaria）、美洲沙漠蝗（Schistocerca americana）和飞蝗（Locusta migratoria）等蝗虫。在这项研究中，我们运用束路追踪、免疫组织化学和细胞内填充等方法，对印度稻蝗视叶及其相关通路的解剖学特征进行了表征。在印度稻蝗中能够识别出其他直翅目物种中已报道的视觉通路的所有区域。免疫组织化学和束路追踪结果显示，视觉通路呈现出相似的结构和连接性，这支持了蝗科昆虫各物种间这些特征的保守性。我们在原脑后部发现了两个新结构，即PS1和PS2，它们有来自视叶的显著神经支配。新结构PS1通过PS1T接受来自髓质的神经支配，PS2通过PS2T接受来自前内侧区（aMe）的神经支配，这两条新通路都是我们识别出来的。
