Wang Yanbin, Li Rong, Wang Chunjing, Sun Ting, Zhang Hongjuan, Zhao Fang, Liu Jiehui, Hao Yuqiong, Xie Xiansheng
Institute of Wheat Research, Shanxi Agricultural University, Linfen, Shanxi, China.
College of Plant Protection, Shanxi Agricultural University, Taigu, Shanxi, China.
Front Microbiol. 2025 Jan 22;16:1517280. doi: 10.3389/fmicb.2025.1517280. eCollection 2025.
is a destructive pest that threatens multiple leguminous crops in China. The intestinal microbiota plays a crucial role in the growth and reproduction of host insects. However, the composition and function of the gut microbiota at different developmental stages remain unclear.
Here, metagenomic sequencing was performed to clarify the gut microbial diversity and function in 2nd-, 3rd-, 4th-, and 5th- instar nymphs (2 N-5 N) and female adults (FAs) of and the effects of vital gut bacteria on development was detected. The gut bacteria have the stage specificity, indicating their function in the development of .
and were the predominant bacteria present during the development of the 2 N-FAs. In addition, the microbial abundances in the 3 N and 4 N guts were significantly greater than those in the others guts. Furthermore, 5 N harbored the abundant microbiota --. The metabolic pathways were significantly enriched from 2 N to FAs. Carbohydrate metabolism, including glycoside hydrolases (GHs) and glycosyl transferases (GTs), occurs throughout the entire developmental stage. Many antibiotic resistance genes (ARGs) were detected from 2 N to FAs. The bacteria from Pseudomonadota and Bacillota presented a broad spectrum of antibiotic resistance. Excitingly, bacteria eliminated by antibiotic treatment were unable to molt normally, and their lifespan was shortened in nymphs, indicating that the gut microbiota had a significant effect on nymph development.
In summary, our results, for the first time, systematically illustrate the abundance and function across the gut microbiota from the different developmental stages of and demonstrate that the genera are crucial during the development of . This study provides the basis for stinkbug management strategies that focus on the pivotal gut microbiota.
是一种具有破坏性的害虫，威胁着中国的多种豆科作物。肠道微生物群在宿主昆虫的生长和繁殖中起着至关重要的作用。然而，不同发育阶段肠道微生物群的组成和功能仍不清楚。
在此，进行了宏基因组测序，以阐明[害虫名称]二龄、三龄、四龄和五龄若虫（2N - 5N）以及雌成虫（FAs）的肠道微生物多样性和功能，并检测重要肠道细菌对发育的影响。肠道细菌具有阶段特异性，表明它们在[害虫名称]发育中的作用。
[细菌名称1]和[细菌名称2]是2N - FAs发育过程中存在的主要细菌。此外，三龄和四龄若虫肠道中的微生物丰度显著高于其他龄期。此外，五龄若虫拥有丰富的微生物群——[微生物群名称]。从二龄到雌成虫，代谢途径显著富集。碳水化合物代谢，包括糖苷水解酶（GHs）和糖基转移酶（GTs），在整个发育阶段都有发生。从二龄到雌成虫检测到许多抗生素抗性基因（ARGs）。变形菌门和厚壁菌门的细菌呈现出广泛的抗生素抗性。令人兴奋的是，经抗生素处理消除的[害虫名称]细菌无法正常蜕皮，若虫寿命缩短，表明肠道微生物群对若虫发育有显著影响。
总之，我们的结果首次系统地阐明了[害虫名称]不同发育阶段肠道微生物群的丰度和功能，并证明[细菌属名称]在[害虫名称]发育过程中至关重要。本研究为以关键肠道微生物群为重点的蝽象管理策略提供了依据。
