Ren Yipeng, Chen Juhong, Fu Siying, Bu Wenjun, Xue Huaijun
Institute of Entomology, College of Life Sciences, Nankai University, Tianjin 300071, PR China.
Comp Biochem Physiol Part D Genomics Proteomics. 2023 Dec;48:101135. doi: 10.1016/j.cbd.2023.101135. Epub 2023 Sep 3.
Insects possess complex and dynamic gut microbial system, which contributes to host nutrient absorption, reproduction, energy metabolism, and protection against stress. However, there are limited data on interactions of host-gut bacterial microbiota through miRNA (microRNA) regulation in a significant pest, Riptortus pedestris. Here, we performed the 16S rRNA amplicon sequencing and small RNA sequencing from the R. pedestris gut under three environmental conditions and antibiotic treatment, suggesting that we obtained a large amount of reads by assembly, filtration and quality control. The 16S rRNA amplicon sequencing results showed that the abundance and diversity of gut bacterial microbiota were significantly changed between antibiotic treatment and other groups, and they are involved in metabolism and biosynthesis-related function based on functional prediction. Furthermore, we identified different numbers of differentially expressed unigenes (DEGs) and differentially expressed miRNAs (DEMs) based on high-quality mappable reads, which were enriched in various immune-related pathways, including Toll-like receptor, RIG-I-like receptor, NOD-like receptor, JAK/STAT, PI3K/Akt, NF-κB, MAPK signaling pathways, and so forth, using GO and KEGG enrichment analysis. Later on, the identified miRNAs and their target genes in the R. pedestris gut were predicted and randomly selected to construct an interaction network. Finally, our study indicated that alterations in the gut bacterial microbiota are significantly positively or negatively associated with DEMs of the Toll/Imd signaling pathway with Pearson correlation analysis. Taken together, the results of our study lay the foundation for further deeply understanding the interactions between the gut microbiota and immune responses in R. pedestris through miRNA regulation, and provide the new basis for pest management in hemipteran pests.
昆虫拥有复杂且动态的肠道微生物系统，该系统有助于宿主的营养吸收、繁殖、能量代谢以及抵御压力。然而，关于重要害虫稻绿蝽中宿主与肠道细菌微生物群通过微小RNA（miRNA）调控相互作用的数据有限。在此，我们在三种环境条件和抗生素处理下对稻绿蝽肠道进行了16S rRNA扩增子测序和小RNA测序，表明通过组装、过滤和质量控制获得了大量读数。16S rRNA扩增子测序结果显示，抗生素处理组与其他组之间肠道细菌微生物群的丰度和多样性发生了显著变化，基于功能预测，它们参与了代谢和生物合成相关功能。此外，基于高质量可映射读数，我们鉴定出了不同数量的差异表达单基因（DEG）和差异表达微小RNA（DEM），通过基因本体论（GO）和京都基因与基因组百科全书（KEGG）富集分析发现，这些差异表达基因和微小RNA富集在各种免疫相关途径中，包括Toll样受体、RIG-I样受体、NOD样受体、JAK/STAT、PI3K/Akt、NF-κB、丝裂原活化蛋白激酶（MAPK）信号通路等。随后，对稻绿蝽肠道中鉴定出的微小RNA及其靶基因进行了预测，并随机选择构建了一个相互作用网络。最后，我们的研究通过Pearson相关性分析表明，肠道细菌微生物群的变化与Toll/Imd信号通路的DEM显著正相关或负相关。综上所述，我们的研究结果为进一步深入了解稻绿蝽中肠道微生物群与免疫反应之间通过miRNA调控的相互作用奠定了基础，并为半翅目害虫的害虫管理提供了新的依据。
