Trybus Wojciech, Trybus Ewa, Obarzanowski Mateusz, Król Teodora
Department of Medical Biology, Jan Kochanowski University of Kielce, Uniwersytecka 7, Kielce, 25-406, Poland.
Department of Oncology, Medical College, Jan Kochanowski University of Kielce, al. IX Wieków Kielc 19a, Kielce, 25-516, Poland.
Sci Rep. 2025 Feb 12;15(1):5252. doi: 10.1038/s41598-025-89847-8.
Cancer diseases are a serious health problem for society, and among them cervical and prostate cancer rank high in terms of mortality. One of the reasons is the phenomenon of drug resistance and side effects accompanying conventional chemo- and radiotherapy. This requires continuous development of alternative treatment methods and searching for new compounds with anti-cancer potential. An example is quinalizarin, which was tested for its anti-cancer potential. The MTT test showed cytotoxic activity of quinalizarin against Hela and DU145 cell lines. Morphological analysis showed nuclear changes typical of apoptosis, which was confirmed by the annexin V/PE test, activation of caspases 3/7 and inhibition of Bcl-2 protein expression. Increased permeability of mitochondrial membranes and ROS generation were demonstrated. Inhibition of cell migration, blocking in the G0/G1 phase, increased number of cells with damaged DNA and an increase in markers of mitotic catastrophe, i.e. micro- and multinucleation including the presence of abnormal mitotic figures were also observed. At the same time, increased autophagy was observed, and preincubation of cells with chloroquine inhibited this process, which contributed to the increased cytotoxicity of quinalizarin towards the tested cells. Quinalizarin has a multidirectional effect based on apoptosis and alternative types of cell death.
癌症疾病是社会面临的一个严重健康问题，其中宫颈癌和前列腺癌的死亡率很高。原因之一是传统化疗和放疗伴随的耐药性和副作用现象。这就需要不断开发替代治疗方法并寻找具有抗癌潜力的新化合物。茜素喹啉就是一个例子，它已被测试其抗癌潜力。MTT试验表明茜素喹啉对Hela和DU145细胞系具有细胞毒性活性。形态学分析显示出典型的凋亡核变化，这通过膜联蛋白V/PE试验、半胱天冬酶3/7的激活以及Bcl-2蛋白表达的抑制得到证实。线粒体膜通透性增加和活性氧生成也得到了证明。还观察到细胞迁移受到抑制、细胞阻滞在G0/G1期、DNA受损的细胞数量增加以及有丝分裂灾难标志物增加，即微核和多核形成，包括异常有丝分裂图像的出现。同时，观察到自噬增加，用氯喹对细胞进行预孵育可抑制这一过程，这导致茜素喹啉对受试细胞的细胞毒性增加。茜素喹啉基于凋亡和其他类型的细胞死亡具有多向作用。
