通过全息弛豫光谱法测定蛋白质的示踪扩散系数：应用于牛血清白蛋白。

Tracer diffusion coefficients of proteins by means of holographic relaxation spectroscopy: application to bovine serum albumin.

作者信息

Arunyawongsakorn U, Johnson C S, Gabriel D A

出版信息

Anal Biochem. 1985 Apr;146(1):265-70. doi: 10.1016/0003-2697(85)90425-7.

Abstract

Holographic relaxation spectroscopy has been used to measure tracer diffusion coefficients for photochromically labeled bovine serum albumin in solutions having total bovine serum albumin concentrations in the range 3.25 to 257 g/liter. In the limit of zero concentration, the diffusion coefficient (20 degrees C, 0.1 M NaCl, 0.05 M Tris, pH 8.0) was found to be (5.9 +/- 0.1) X 10(-7) cm2/s and the initial slope was zero. The concentration dependence of the diffusion coefficient was not significantly affected by the fraction of protein molecules which were labeled. Holographic relaxation spectroscopy permits rapid, accurate determination of tracer diffusion coefficients for proteins in mixtures.

摘要

全息弛豫光谱法已被用于测量光致变色标记的牛血清白蛋白在总牛血清白蛋白浓度范围为3.25至257克/升的溶液中的示踪扩散系数。在零浓度极限下，发现扩散系数（20℃，0.1M NaCl，0.05M Tris，pH 8.0）为（5.9±0.1）×10⁻⁷平方厘米/秒，且初始斜率为零。标记的蛋白质分子比例对扩散系数的浓度依赖性没有显著影响。全息弛豫光谱法能够快速、准确地测定混合物中蛋白质的示踪扩散系数。