Wakeham W A, Salpadoru N H, Caro C G
Atherosclerosis. 1976 Nov-Dec;25(2-3):225-35. doi: 10.1016/0021-9150(76)90029-0.
A new technique is described for the measurement of the self-diffusion coefficients of protein macromolecules in solution. The method makes use of the phenomenon of Taylor dispersion of a solute introduced into a solvent flowing in the laminar regime through a tube of circular section. Results are reported for the self-diffusion coefficient of cholesterol associated with lipoprotein molecules in dogs' serum at pH 7.4 in the temperature range 18-37 degrees C. The diffusivity of bovine serum albumin in serum has also been studied as a function of temperature at pH 7.4 and 4.7. In the more basic solution, measurements of the diffusivity as a function of protein concentration substantially agree with earlier work. For all the systems studied the diffusivity varies rapidly with temperature. The pH of the solution, in the case of bovine serum albumin, also has a significant effect on the diffusivity of the macromolecule. The latter observation is related to the amount of water bound to the protein molecule in solution.
本文描述了一种用于测量溶液中蛋白质大分子自扩散系数的新技术。该方法利用了溶质在层流状态下通过圆形截面管在溶剂中流动时的泰勒分散现象。报告了在18 - 37摄氏度、pH 7.4条件下狗血清中与脂蛋白分子相关的胆固醇自扩散系数的结果。还研究了牛血清白蛋白在血清中的扩散系数随温度在pH 7.4和4.7条件下的变化情况。在碱性更强的溶液中，扩散系数随蛋白质浓度变化的测量结果与早期工作基本一致。对于所有研究的系统，扩散系数随温度变化迅速。对于牛血清白蛋白，溶液的pH值对大分子的扩散系数也有显著影响。后一观察结果与溶液中与蛋白质分子结合的水量有关。
