Fried V A, Ando M E, Bell A J
Anal Biochem. 1985 Apr;146(1):271-6. doi: 10.1016/0003-2697(85)90426-9.
A fluorescent protein assay was described wherein an isocratic high-performance liquid chromatography system was used to separate the o-phthaldialdehyde-derivatized proteins from interfering components. Using a small TSK guard column equilibrated in 0.1% sodium dodecyl sulfate, it was demonstrated that all proteins and peptides examined, containing more than 22 residues, coelute in the excluded volume and were resolved from fluorescent signals contributed by commonly used reagents. The assay was linear over a useful range of 3 ng to 1 microgram of protein and required less than 15 microliter of sample.
描述了一种荧光蛋白测定法，其中使用等度高效液相色谱系统从干扰成分中分离邻苯二甲醛衍生化的蛋白质。使用在0.1%十二烷基硫酸钠中平衡的小型TSK保护柱，结果表明，所有检测的含有超过22个残基的蛋白质和肽在排阻体积中共洗脱，并与常用试剂产生的荧光信号分离。该测定法在3 ng至1微克蛋白质的有效范围内呈线性，且所需样品量少于15微升。
