Hammer K D, Nagel K
Anal Biochem. 1986 Jun;155(2):308-14. doi: 10.1016/0003-2697(86)90439-2.
An automated assay for measuring nanogram and subnanogram quantities of protein in microliter samples was developed with the fluorometric reagent omicron-phthaldialdehyde/mercaptoethanol. Low molecular weight interfering substances were separated within the analysis by gel filtration. The technique allowed measurement of biological fluids without any sample pretreatment. The method presented proved to be linear within the range 1.2 ng to 1.4 microgram with a standard deviation of +/- 4.2%. The minimum detectable protein concentration was 1 microgram/liter. Special care was taken to prevent any determination errors caused by losses of protein during adsorption to surfaces. The simple analytical apparatus constructed can be used for field studies and ran several weeks when continuously used for seawater analysis aboard ship.
利用荧光试剂邻苯二甲醛/巯基乙醇开发了一种用于测量微升样品中纳克和亚纳克级蛋白质含量的自动化检测方法。在分析过程中，通过凝胶过滤分离低分子量干扰物质。该技术无需对样品进行任何预处理即可测量生物体液。所提出的方法在1.2纳克至1.4微克范围内呈线性，标准偏差为±4.2%。最低可检测蛋白质浓度为1微克/升。特别注意防止蛋白质吸附到表面时损失而导致的任何测定误差。所构建的简单分析仪器可用于现场研究，在船上连续用于海水分析时可运行数周。
