一种通过碘氧化测定异羟肟酸的比色法。

A colorimetric method for the determination of hydroxamic acid by iodine oxidation.

作者信息

Kobashi K, Sakaguchi K, Takebe S, Hosaka K

出版信息

Anal Biochem. 1985 Apr;146(1):7-12. doi: 10.1016/0003-2697(85)90387-2.

Abstract

A new colorimetric method for the determination of hydroxamic acid is described. Hydroxamic acid was oxidized quantitatively by iodine to produce nitrous acid, which was thereafter determined according to the diazocoupling reaction. This method is sensitive to as little as 5 nmol of hydroxamic acid, and the calibration curve is linear up to 50 nmol. Using this method, acyl-CoAs were determined after conversion to hydroxamic acid by the addition of hydroxylamine. The present method is applicable to the determination of free fatty acids which are activated by acyl-CoA synthetase.

摘要

本文描述了一种测定异羟肟酸的新比色法。异羟肟酸被碘定量氧化生成亚硝酸，然后根据重氮偶合反应对其进行测定。该方法对低至5 nmol的异羟肟酸敏感，校准曲线在50 nmol范围内呈线性。使用该方法，通过加入羟胺将酰基辅酶A转化为异羟肟酸后进行测定。本方法适用于测定由酰基辅酶A合成酶激活的游离脂肪酸。