体内证据表明，酰基辅酶A可调节大肠杆菌全局转录因子FadR与DNA的结合。
In vivo evidence that acyl coenzyme A regulates DNA binding by the Escherichia coli FadR global transcription factor.
作者信息
Cronan J E
机构信息
Department of Microbiology, University of Illinois, Urbana 61801, USA.
出版信息
J Bacteriol. 1997 Mar;179(5):1819-23. doi: 10.1128/jb.179.5.1819-1823.1997.
Abstract
In vitro experiments point to fatty acyl coenzymes A (acyl-CoAs) rather than unesterified fatty acids as the small-molecule ligands regulating DNA binding by the FadR protein of Escherichia coli. To provide an in vivo test of this specificity, unesterified fatty acids were generated within the cellular cytosol. These fatty acids were found to be efficient modulators of FadR action only when the acids could be converted to acyl-CoAs.
摘要
体外实验表明，作为调节大肠杆菌FadR蛋白与DNA结合的小分子配体是脂肪酰辅酶A（酰基辅酶A）而非未酯化脂肪酸。为了对这种特异性进行体内测试，在细胞胞质溶胶中产生了未酯化脂肪酸。结果发现，只有当这些脂肪酸能够转化为酰基辅酶A时，它们才是FadR作用的有效调节剂。
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