Effects of exogenous melatonin on the growth and photosynthetic characteristics of tomato seedlings under saline-alkali stress.

Saline-alkali stress is a major abiotic stress factor that adversely affects the growth, development, and yield of crops by disrupting ion homeostasis, osmotic balance, and metabolic processes. This study was designed to explore the alleviating effect of melatonin on the growth and development of tomato plants under saline-alkali stress conditions and to screen for optimal concentrations to alleviate the stress. Tomato variety 'Condine Red' was used as the test material, and a total of six treatments were designed including no saline-alkali stress and no melatonin spray as control (CK), and foliar spraying of 0, 50, 100, 150, and 200 µmol·L melatonin under saline-alkali stress (75 mmol·L), which were used to determine the growth and photosynthetic characteristics of tomato plants. The results showed that saline-alkali stress significantly inhibited plant height, stem diameter, root activity and biomass accumulation, significantly reduced the chlorophyll content of tomato leaves and the efficiency of photosynthetic electron transfer from primary quinone receptor QA to secondary quinone receptor QB, and caused significant deformation of the fast chlorophyll fluorescence induced kinetic curve (OJIP), inhibiting photosynthesis. Exogenous melatonin could improve tomato tolerance to saline-alkali stress, and the effect depended on the concentration. In this experiment, treatment with 100 µmol·L melatonin showed the strongest positive effect on the growth of tomato plants under saline-alkali stress according to the comprehensive evaluation of principal components. In addition, changes in photosynthetic chlorophyll fluorescence parameters and chlorophyll fluorescence induction curves after melatonin treatment highlighted that melatonin could improve the response of the photosynthetic system to saline-alkali stress by enhancing quenching of excess excitation energy and protecting the photosynthetic electron transport system. Collectively, exogenous melatonin pretreatment increased root activity, chlorophyll content and improved photosystem processes, thereby alleviating tomato growth under saline-alkali stress. The results of this study lay the foundation for the practical application of melatonin in saline-alkali stress.