Naig Princess Joy, Kuo Zih-Yin, Chung Min-Fan, Chen Chih-Hao, Wang Chi-Yun, Hung Kuo-Yung
Department of Biomedical Engineering and Medical Devices, Ming Chi University of Technology, New Taipei City 24301, Taiwan.
Research Center for Intelligent Medical Devices, Ming Chi University of Technology, New Taipei City 24301, Taiwan.
Polymers (Basel). 2025 Jan 21;17(3):256. doi: 10.3390/polym17030256.
In regenerative periodontal treatment, barrier membranes restore periodontal support and aid tissue healing, but slow hard tissue regeneration can disrupt healing and cause tooth instability. This study aimed to fabricate a periodontal membrane through electrospinning poly(L-lactide-co-D, L-lactide) with varying β-tricalcium phosphate (β-TCP) percentages (0%, 10%, 30%, and 40%) treated with hyaluronic acid to enhance bone regeneration in alveolar bone defects. Their ability to promote biomimetic mineralization was characterized using field emission scanning electron microscopy (FESEM) analysis, wettability, and mechanical properties. Biocompatibility and osteogenic differentiation were evaluated by examining BMSCs' behavior. In vivo, the PLA/β-TCP membrane's potential to promote bone regeneration was assessed through CT imaging and histological examination. FESEM analysis revealed β-TCP agglomerations within PLA fibers, increasing tensile strength. Water contact angle measurements showed better wettability and higher cell viability after hyaluronic acid treatment, indicating non-cytotoxicity. Membranes with 10% and 30% (/) β-TCP significantly enhanced cellular activities, including proliferation and osteogenic differentiation. Animal tests showed a significant bone growth rate increase to 28.9% in the experimental group compared to 24.9% with the commercial product Epi-Guide after three months. Overall, PLA with 30% β-TCP optimally promoted periodontal hard tissue repair and potentially enhanced bone regeneration.
在再生性牙周治疗中，屏障膜可恢复牙周支持并促进组织愈合，但硬组织再生缓慢会干扰愈合并导致牙齿不稳定。本研究旨在通过静电纺丝制备含不同比例（0%、10%、30%和40%）β-磷酸三钙（β-TCP）的聚（L-丙交酯-co-D，L-丙交酯）牙周膜，并用透明质酸处理以增强牙槽骨缺损中的骨再生。通过场发射扫描电子显微镜（FESEM）分析、润湿性和力学性能来表征其促进仿生矿化的能力。通过检测骨髓间充质干细胞（BMSCs）的行为来评估生物相容性和成骨分化。在体内，通过CT成像和组织学检查评估聚乳酸/β-TCP膜促进骨再生的潜力。FESEM分析显示PLA纤维内有β-TCP团聚，拉伸强度增加。水接触角测量表明，透明质酸处理后润湿性更好，细胞活力更高，表明无细胞毒性。含10%和30%（/）β-TCP的膜显著增强了细胞活性，包括增殖和成骨分化。动物试验显示，三个月后，实验组的骨生长率显著提高至28.9%，而使用商业产品Epi-Guide的对照组为24.9%。总体而言，含30%β-TCP的聚乳酸能最佳地促进牙周硬组织修复，并可能增强骨再生。
