Jones Robert S, Pride Morgan Annina, Kumar Dhiraj
Division of Pediatric Dentistry, Department of Developmental and Surgical Sciences, School of Dentistry, University of Minnesota, Minneapolis, MN 55455, USA.
North Carolina Agricultural and Technical State University, Greensboro, NC 27411, USA.
Int J Mol Sci. 2025 Jan 31;26(3):1242. doi: 10.3390/ijms26031242.
An over-the-counter vasoconstrictive nasal solution with oxymetazoline (NS-OXY, 0.05%) has the potential to be used as a dental pulpal hemostatic medicament. A molecular engineering approach examined NS-OXY and its molecular constituent's antimicrobial and blood biomass removal efficacy.
An ex vivo cavity model was developed where standardized prepared teeth were exposed overnight to a model dentinal caries pathogen, , and then exposed to sheep's blood for 10 min, which simulated a pulpal exposure. Cavity preparations were rinsed with OXY (0.05%), benzalkonium chloride (BKC-0.025%), NS-OXY (with OXY-0.05% and BKC), ferric sulfate (20%;ViscoStat, FS), and distilled water (DI). For examining the bactericidal effect of NS-OXY, a disk diffusion antimicrobial assay was used where was grown (20 h) on brain heart infusion (BHI) w/0.5% glucose agar plates and exposed to the treatment groups.
NS-OXY-treated samples had a lower residual bacterial or blood biomass than FS ( = 0.003). The diffusion test showed that NS-OXY, BKC, and FS had zones of inhibition greater than 10 mm, with NS-OXY having higher activity against than FS ( = 0.0002), but lower than BKC ( = 0.0082).
NS-OXY may be considered as a dental hemostatic agent after traumatic and carious pulpal exposure owing to NS-OXY's antimicrobial and vasoconstrictive properties.
一种含羟甲唑啉的非处方血管收缩性鼻腔溶液（NS - OXY，0.05%）有潜力用作牙髓止血药物。一种分子工程方法研究了NS - OXY及其分子成分的抗菌和去除血液生物量的功效。
建立了一个体外洞腔模型，将标准化制备的牙齿暴露于模型牙本质龋病原体过夜，然后暴露于羊血10分钟，模拟牙髓暴露。用OXY（0.05%）、苯扎氯铵（BKC - 0.025%）、NS - OXY（含OXY - 0.05%和BKC）、硫酸铁（20%；ViscoStat，FS）和蒸馏水（DI）冲洗洞腔制剂。为了研究NS - OXY的杀菌效果，采用纸片扩散抗菌试验，将 在含0.5%葡萄糖的脑心浸液（BHI）琼脂平板上培养（20小时），然后暴露于各治疗组。
NS - OXY处理的样本残留细菌或血液生物量低于FS（P = 0.003）。扩散试验表明，NS - OXY、BKC和FS的抑菌圈大于10毫米，NS - OXY对 的活性高于FS（P = 0.0002），但低于BKC（P = 0.0082）。
由于NS - OXY的抗菌和血管收缩特性，在创伤性和龋源性牙髓暴露后，NS - OXY可被视为一种牙科止血剂。
