Delrue Charlotte, De Bruyne Sander, Speeckaert Marijn M
Department of Nephrology, Ghent University Hospital, 9000 Ghent, Belgium.
Department of Diagnostic Sciences, Ghent University, 9000 Ghent, Belgium.
Diagnostics (Basel). 2025 Feb 4;15(3):368. doi: 10.3390/diagnostics15030368.
Attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy has shown significant promise in the context of liquid biopsy, offering a potential tool for cancer diagnostics. Unlike traditional tissue biopsies, which may not fully capture the clonal heterogeneity of tumors, liquid biopsy reflects the dynamic state of the disease and its progression more comprehensively. Biofluids such as serum and plasma are low-cost, minimally invasive diagnostic media with well-established clinical uses. This review assesses the use of ATR-FTIR spectroscopy to detect biochemical changes in biofluids linked to various malignancies, including breast, ovarian, endometrial, prostate, bladder, kidney, pancreatic, colorectal, hepatic, esophageal, gastric, lung, and brain cancers. While ATR-FTIR offers the advantages of rapid, minimally invasive detection and real-time disease monitoring, its integration into clinical practice faces challenges, particularly in terms of reproducibility due to variability in sample preparation, spectral acquisition, and data processing. The translation of ATR-FTIR into routine diagnostics will require validation through large-scale cohort studies and multicenter trials to ensure its clinical reliability and effectiveness.
衰减全反射傅里叶变换红外光谱（ATR-FTIR）在液体活检领域已显示出巨大的前景，为癌症诊断提供了一种潜在工具。与传统组织活检不同，传统组织活检可能无法完全捕捉肿瘤的克隆异质性，而液体活检能更全面地反映疾病的动态状态及其进展。血清和血浆等生物流体是低成本、微创的诊断介质，具有成熟的临床应用。本综述评估了ATR-FTIR光谱在检测与各种恶性肿瘤相关的生物流体生化变化中的应用，这些恶性肿瘤包括乳腺癌、卵巢癌、子宫内膜癌、前列腺癌、膀胱癌、肾癌、胰腺癌、结直肠癌、肝癌、食管癌、胃癌、肺癌和脑癌。虽然ATR-FTIR具有快速、微创检测和实时疾病监测的优点，但其融入临床实践面临挑战，特别是由于样品制备、光谱采集和数据处理的可变性导致的重现性问题。将ATR-FTIR转化为常规诊断需要通过大规模队列研究和多中心试验进行验证，以确保其临床可靠性和有效性。
