Berry J P, Poupon M F, Judde J C, Galle P
Ann Clin Lab Sci. 1985 Mar-Apr;15(2):109-20.
Some nickel compounds (Ni3S2,Ni) induce tumours in muscle, while others have no effect (NiO). It has been suggested that the carcinogenicity of nickel is related to its penetrating power (phagocytosis) in transformed cells. The penetration of various nickel salts into cultured rhabdomyosarcoma cells (RhC) was studied. Electron microscopy and microanalysis were used to study the ultrastructure and intracellular localization of nickel in ultra-thin sections. Nickel subsulfide (Ni3S2) and nickel oxide (NiO) penetrated into cells and were concentrated in vacuoles, exhibiting a particular affinity for membrane structure. They subsequently appeared to be eliminated in the extracellular medium. Colloidal nickel and iron carbonyl, on the other hand, did not penetrate these cells. Various tumoral and normal cells were compared for their ability to phagocytose Ni3S2; it was found that these compounds penetrated only RhC and macrophages. In vivo studies have demonstrated the various carcinogenic properties of nickel and two of its salts. Comparison with in vitro results suggests that the phagocytosis of nickel compounds is not directly related to eventual induction of a tumour. No nuclear localization could be detected, but a mechanism for concentration and elimination of these compounds, and especially for rhabdomyosarcoma tumour cells, was suggested.
一些镍化合物（Ni3S2、Ni）可诱发肌肉肿瘤，而其他一些则无此作用（NiO）。有人提出镍的致癌性与其在转化细胞中的穿透能力（吞噬作用）有关。研究了各种镍盐对培养的横纹肌肉瘤细胞（RhC）的穿透情况。利用电子显微镜和微量分析研究了超薄切片中镍的超微结构和细胞内定位。硫化亚镍（Ni3S2）和氧化镍（NiO）可穿透细胞并聚集在液泡中，对膜结构表现出特殊亲和力。它们随后似乎在细胞外介质中被清除。另一方面，胶体镍和羰基铁不能穿透这些细胞。比较了各种肿瘤细胞和正常细胞吞噬Ni3S2的能力；发现这些化合物仅能穿透RhC和巨噬细胞。体内研究证实了镍及其两种盐类具有不同的致癌特性。与体外结果比较表明，镍化合物的吞噬作用与最终诱发肿瘤并无直接关系。未检测到镍在细胞核中的定位，但提出了这些化合物，尤其是横纹肌肉瘤肿瘤细胞中镍的聚集和清除机制。
