Lee Sue-Kei, Keng Jing-Wen, Yon Jessica-Ai-Lyn, Mai Chun-Wai, Lim Heng-Chee, Chow Sek-Chuen, Akowuah Gabriel Akyirem, Liew Kai Bin, Lee Siew-Keah, Marriott Philip J, Chew Yik-Ling
Faculty of Pharmaceutical Sciences, UCSI University, Kuala Lumpur 56000, Malaysia.
Centre for Cancer and Stem Cell Research, Institute for Research, Development and Innovation (IRDI), IMU University, Kuala Lumpur 57000, Malaysia.
Plants (Basel). 2025 Jan 24;14(3):362. doi: 10.3390/plants14030362.
To study (CA) (Linnaeus) Roxburgh's effectiveness towards atopic dermatitis (AD), CA leaf extracts were prepared using three methanol-based extraction solvent systems. Bioactive constituents were characterized and quantified via high-performance liquid chromatography with diode array detection. Antioxidant properties and antimicrobial activities against , a major AD exacerbation factor, were assessed. Four polyphenols (two flavonoids, two anthraquinones) beneficial in AD control were detected (rhein > aloe-emodin > astragalin > kaempferol). The 75% / MeOH/water extract had the most polyphenols and the best antioxidant profile (total phenolic content, total flavonoid content, 2,2-diphenyl-1-picrylhydrazyl-hydrate radical scavenging activity, ascorbic acid equivalent antioxidant capacity), with excellent inhibition (minimum inhibitory concentration = 0.625 mg/mL; minimum bactericidal concentration = 1.25 mg/mL). Hence, it was selected for the in vitro examination of cytotoxicity and wound healing activity towards human epidermal keratinocyte cells using a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2h-tetrazolium bromide (MTT) assay and wound scratch assay. The extract showed no cytotoxicity (IC > 100 µg/mL) without significant reduction in cell viability up to 200 µg/mL compared to the vehicle control. An amount of 50 μg/mL extract concentration showed the best wound-healing activity ( < 0.05), with a cell migration rate of 5.89 ± 0.80 µm/h over 96 h post-treatment. Such antioxidant, antimicrobial, and wound-healing activities suggest CA and its polyphenols to be promising natural, long-term AD remedies for skin health.
为研究刺蒺藜（(CA) (Linnaeus) Roxburgh）对特应性皮炎（AD）的疗效，使用三种基于甲醇的提取溶剂系统制备了刺蒺藜叶提取物。通过带二极管阵列检测的高效液相色谱对生物活性成分进行了表征和定量。评估了其抗氧化性能以及对AD加重的主要因素金黄色葡萄球菌的抗菌活性。检测到四种对AD控制有益的多酚（两种黄酮类化合物、两种蒽醌）（大黄酸＞芦荟大黄素＞黄芪甲苷＞山奈酚）。75%甲醇/水提取物的多酚含量最高，抗氧化性能最佳（总酚含量、总黄酮含量、2,2-二苯基-1-苦基肼基水合物自由基清除活性、抗坏血酸当量抗氧化能力），对金黄色葡萄球菌具有优异的抑制作用（最低抑菌浓度=0.625 mg/mL；最低杀菌浓度=1.25 mg/mL）。因此，使用3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2h-溴化四唑（MTT）法和伤口划痕试验，选择该提取物对人表皮角质形成细胞进行细胞毒性和伤口愈合活性的体外检测。与赋形剂对照相比，该提取物在高达200 µg/mL时无细胞毒性（IC＞100 µg/mL），细胞活力无显著降低。50 μg/mL的提取物浓度显示出最佳的伤口愈合活性（P＜0.05），处理后96小时的细胞迁移率为5.89±0.80 µm/h。这些抗氧化、抗菌和伤口愈合活性表明刺蒺藜及其多酚有望成为促进皮肤健康的天然、长期AD治疗药物。
