Ito H
Auris Nasus Larynx. 1979;6(2):97-103. doi: 10.1016/s0385-8146(79)80013-9.
The prinicpal methods presently being applied to distinction of the subpopulations of lymphocytes consist in the E-rosette method for T cells and the EAC-rosette method for B cells. However, it has been learned that other cells have the ability to form rosettes. B cells are employed in various experiments due to the explicitly verifiable immunoglobulin on the surface of lymphocytes. However, as the fluorescent antibody method adopted as the method for verification has a number of difficulties, we tried to determine B cells by utilizing protein A, by combining protein A with SRBC (sheep red blood cells) and forming rosettes, both in human peripheral blood and in human intratonsillar lymphocytes. As a result, it was noted that lymphocytes indicating positivity existed at a level of 15-20% in human blood and 40-45% in the tonsil.
目前用于区分淋巴细胞亚群的主要方法包括用于T细胞的E花环法和用于B细胞的EAC花环法。然而，现已了解到其他细胞也有形成花环的能力。由于淋巴细胞表面存在可明确验证的免疫球蛋白，B细胞被用于各种实验。然而，由于用作验证方法的荧光抗体法存在诸多困难，我们尝试通过利用蛋白A来确定B细胞，即将蛋白A与绵羊红细胞（SRBC）结合形成花环，应用于人类外周血和人类扁桃体内部的淋巴细胞。结果发现，呈阳性的淋巴细胞在人类血液中的比例为15% - 20%，在扁桃体中的比例为40% - 45%。
