Yata J, Tsukimoto I, Tachibana T
Clin Exp Immunol. 1973 Jul;14(3):319-26.
Human lymphocytes from various lymphoid tissues were studied for the relationship between the existence of HTL (human thymus-lymphoid tissue) antigen, and binding of sheep erythrocytes (E) or sheep erythrocyte–antibody-complement complexes (EA(IgM)C43). E adhered to the majority of thymus lymphocytes and formed rosettes. These lymphocytes were shown to be HTL antigen positive by immunofluorescence performed simultaneously. In the peripheral lymphoid tissues, 10–30% of lymphocytes formed E rosettes and almost all E rosette-forming lymphocytes were HTL antigen positive. Conversely HTL antigen-negative cells did not form E rosettes. In contrast, the cells binding EA(IgM)C43 were always HTL antigen negative. There were very few HTL antigen-positive or rosette-forming lymphocytes either with E or EA(IgM)C43 in bone marrow. From these data we conclude that E-rosette-forming and HTL antigen-positive lymphocytes are of thymus origin and EA(IgM)C43-rosette-forming cells are not thymus-dependent cells.
对来自各种淋巴组织的人类淋巴细胞进行了研究，以探讨人类胸腺淋巴细胞（HTL）抗原的存在与绵羊红细胞（E）或绵羊红细胞-抗体-补体复合物（EA（IgM）C43）结合之间的关系。E粘附于大多数胸腺淋巴细胞并形成玫瑰花结。通过同时进行的免疫荧光显示，这些淋巴细胞为HTL抗原阳性。在周围淋巴组织中，10%-30%的淋巴细胞形成E玫瑰花结，几乎所有形成E玫瑰花结的淋巴细胞均为HTL抗原阳性。相反，HTL抗原阴性细胞不形成E玫瑰花结。相比之下，结合EA（IgM）C43的细胞始终为HTL抗原阴性。骨髓中无论是与E还是与EA（IgM）C43结合的HTL抗原阳性或形成玫瑰花结的淋巴细胞都非常少。从这些数据我们得出结论，形成E玫瑰花结和HTL抗原阳性的淋巴细胞起源于胸腺，而形成EA（IgM）C43玫瑰花结的细胞不是胸腺依赖性细胞。
