• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

人脐带间充质干细胞外泌体-EGR1微针贴片通过调节氧化应激和线粒体自噬对心肌梗死/再灌注损伤的心脏保护作用

Cardioprotective effects of hUCMSCs-Exosi-EGR1 MN patch in MI/RI by modulating oxidative stress and mitophagy.

作者信息

Huang Chen, Zhang Xun, Wu Shi-Xiong, Chang Qing, Zheng Zhi-Kun, Xu Jing

机构信息

Department of Cardiovascular Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450000, China.

出版信息

Mater Today Bio. 2025 Jan 19;31:101500. doi: 10.1016/j.mtbio.2025.101500. eCollection 2025 Apr.

DOI:10.1016/j.mtbio.2025.101500
PMID:39944530
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11815289/
Abstract

In an effort to address the detrimental effects of myocardial ischemia/reperfusion injury (MI/RI), this study introduces a novel therapeutic strategy that involves a microneedle (MN) patch loaded with exosomes derived from human umbilical cord mesenchymal stem cells (hUCMSCs) and carrying small interfering RNA (siRNA) targeting early growth response-1 (EGR1). By delivering hUCMSCs-derived exosomes containing EGR1 siRNA (hUCMSCs-Exosi-EGR1), the MN patch demonstrated promising cardioprotective effects in both and models of MI/RI. The results highlighted the efficacy of Exosi-EGR1 in enhancing cardiomyocyte viability, attenuating oxidative stress levels, and fostering mitophagy regulation. Moreover, the Exosi-EGR1 MN patch exhibited excellent mechanical properties and sustained drug release characteristics when embedded in a Gelatin methacryloyl (GelMA) matrix. Noteworthy outcomes from experiments included significant improvements in cardiac function, reduced cardiac fibrosis, and decreased apoptosis rates in MI/RI mice, emphasizing the potential therapeutic value of the Exosi-EGR1 MN patch in mitigating MI/RI through modulation of oxidative stress and mitophagy mechanisms.

摘要

为了应对心肌缺血/再灌注损伤（MI/RI）的有害影响，本研究引入了一种新的治疗策略，即使用一种微针（MN）贴片，该贴片负载有人脐带间充质干细胞（hUCMSCs）来源的外泌体，并携带靶向早期生长反应-1（EGR1）的小干扰RNA（siRNA）。通过递送含有EGR1 siRNA的hUCMSCs来源的外泌体（hUCMSCs-Exosi-EGR1），MN贴片在MI/RI的体内和体外模型中均显示出有前景的心脏保护作用。结果突出了Exosi-EGR1在提高心肌细胞活力、减轻氧化应激水平和促进线粒体自噬调节方面的功效。此外，当嵌入甲基丙烯酸明胶（GelMA）基质中时，Exosi-EGR1 MN贴片表现出优异的机械性能和持续的药物释放特性。体内实验的显著结果包括MI/RI小鼠的心脏功能显著改善、心脏纤维化减轻和凋亡率降低，强调了Exosi-EGR1 MN贴片通过调节氧化应激和线粒体自噬机制减轻MI/RI的潜在治疗价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1d3/11815289/d99dc5579e40/mmcfigs3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1d3/11815289/0240674d85b8/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1d3/11815289/a18e028cdebe/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1d3/11815289/706c213f2653/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1d3/11815289/be99538c6527/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1d3/11815289/453b4123787c/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1d3/11815289/116aed5d966c/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1d3/11815289/e88beb37ddfa/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1d3/11815289/2a4d093b3c92/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1d3/11815289/fb4d57e93806/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1d3/11815289/2a3dcf39ed52/mmcfigs1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1d3/11815289/e87064836ecc/mmcfigs2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1d3/11815289/d99dc5579e40/mmcfigs3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1d3/11815289/0240674d85b8/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1d3/11815289/a18e028cdebe/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1d3/11815289/706c213f2653/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1d3/11815289/be99538c6527/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1d3/11815289/453b4123787c/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1d3/11815289/116aed5d966c/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1d3/11815289/e88beb37ddfa/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1d3/11815289/2a4d093b3c92/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1d3/11815289/fb4d57e93806/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1d3/11815289/2a3dcf39ed52/mmcfigs1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1d3/11815289/e87064836ecc/mmcfigs2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1d3/11815289/d99dc5579e40/mmcfigs3.jpg

相似文献

1
Cardioprotective effects of hUCMSCs-Exosi-EGR1 MN patch in MI/RI by modulating oxidative stress and mitophagy.人脐带间充质干细胞外泌体-EGR1微针贴片通过调节氧化应激和线粒体自噬对心肌梗死/再灌注损伤的心脏保护作用
Mater Today Bio. 2025 Jan 19;31:101500. doi: 10.1016/j.mtbio.2025.101500. eCollection 2025 Apr.
2
NGF nanoparticles enhance the potency of transplanted human umbilical cord mesenchymal stem cells for myocardial repair.NGF 纳米颗粒增强了移植的人脐带间充质干细胞修复心肌的效力。
Am J Physiol Heart Circ Physiol. 2021 May 1;320(5):H1959-H1974. doi: 10.1152/ajpheart.00855.2020. Epub 2021 Mar 26.
3
Gelatin methacryloyl microneedle loaded with 3D-MSC-Exosomes for the protection of ischemia-reperfusion.明胶甲基丙烯酰微针负载 3D-MSC-Exosomes 用于保护缺血再灌注损伤。
Int J Biol Macromol. 2024 Aug;275(Pt 1):133336. doi: 10.1016/j.ijbiomac.2024.133336. Epub 2024 Jun 25.
4
DanShen Decoction targets miR-93-5p to provide protection against MI/RI by regulating the TXNIP/NLRP3/Caspase-1 signaling pathway.丹参饮通过调控 TXNIP/NLRP3/Caspase-1 信号通路靶向 miR-93-5p 对心肌缺血再灌注损伤发挥保护作用。
Phytomedicine. 2024 Dec;135:156225. doi: 10.1016/j.phymed.2024.156225. Epub 2024 Nov 8.
5
Intramyocardial injected human umbilical cord-derived mesenchymal stem cells (HucMSCs) contribute to the recovery of cardiac function and the migration of CD4 T cells into the infarcted heart via CCL5/CCR5 signaling.心肌内注射人脐带间充质干细胞（HucMSCs）通过 CCL5/CCR5 信号促进心脏功能的恢复和 CD4 T 细胞向梗死心脏的迁移。
Stem Cell Res Ther. 2022 Jun 11;13(1):247. doi: 10.1186/s13287-022-02914-z.
6
Microneedle Patch Loaded with Exosomes Containing MicroRNA-29b Prevents Cardiac Fibrosis after Myocardial Infarction.载有 microRNA-29b 的外泌体的微针贴片可预防心肌梗死后的心脏纤维化。
Adv Healthc Mater. 2023 May;12(13):e2202959. doi: 10.1002/adhm.202202959. Epub 2023 Feb 5.
7
Galunisertib-Loaded Gelatin Methacryloyl Hydrogel Microneedle Patch for Cardiac Repair after Myocardial Infarction.载姜黄素明胶甲基丙烯酰水凝胶微针贴片用于心肌梗死后的心脏修复。
ACS Appl Mater Interfaces. 2022 Sep 14;14(36):40491-40500. doi: 10.1021/acsami.2c05352. Epub 2022 Aug 29.
8
Pubescenoside D Ameliorates Myocardial Ischemia-Reperfusion Injury via Preventing the Dissociation of HK2 and Promoting Mitophagy by Targeting GSK-3β.毛蕊花糖苷D通过靶向糖原合成酶激酶-3β防止己糖激酶2解离并促进线粒体自噬来改善心肌缺血-再灌注损伤。
Phytother Res. 2025 Mar;39(3):1578-1591. doi: 10.1002/ptr.8434. Epub 2025 Feb 6.
9
Exosomes from umbilical cord-derived mesenchymal stem cells combined with gelatin methacryloyl inhibit vein graft restenosis by enhancing endothelial functions.脐带间充质干细胞来源的外泌体与明胶甲基丙烯酰结合通过增强内皮功能抑制静脉移植物再狭窄。
J Nanobiotechnology. 2023 Oct 18;21(1):380. doi: 10.1186/s12951-023-02145-1.
10
[Effects of gelatin methacrylate anhydride hydrogel loaded with small extracellular vesicles derived from human umbilical cord mesenchymal stem cells in the treatment of full-thickness skin defect wounds in mice].[载有人脐带间充质干细胞来源的小细胞外囊泡的甲基丙烯酸酐明胶水凝胶对小鼠全层皮肤缺损创面的治疗作用]
Zhonghua Shao Shang Yu Chuang Mian Xiu Fu Za Zhi. 2024 Apr 20;40(4):323-332. doi: 10.3760/cma.j.cn501225-20231218-00248.

引用本文的文献

1
Bioengineered microneedles and nanomedicine as therapeutic platform for tissue regeneration.生物工程微针和纳米药物作为组织再生的治疗平台。
J Nanobiotechnology. 2025 Aug 19;23(1):573. doi: 10.1186/s12951-025-03623-4.

本文引用的文献

1
Nanoparticle-Patch System for Localized, Effective, and Sustained miRNA Administration into Infarcted Myocardium to Alleviate Myocardial Ischemia-Reperfusion Injury.用于将微小RNA局部、有效且持续地递送至梗死心肌以减轻心肌缺血-再灌注损伤的纳米颗粒贴片系统。
ACS Nano. 2024 Jul 17. doi: 10.1021/acsnano.3c08811.
2
miR-124-3p downregulates EGR1 to suppress ischemia-hypoxia reperfusion injury in human iPS cell-derived cardiomyocytes.miR-124-3p 通过下调 EGR1 抑制人诱导多能干细胞源性心肌细胞缺血缺氧再灌注损伤。
Sci Rep. 2024 Jun 27;14(1):14811. doi: 10.1038/s41598-024-65373-x.
3
Layer-by-layer microneedle patch with antibacterial and antioxidant dual activities for accelerating bacterial-infected wound healing.
具有抗菌和抗氧化双重活性的层层微针贴片，加速细菌感染性伤口愈合。
Colloids Surf B Biointerfaces. 2023 Nov;231:113569. doi: 10.1016/j.colsurfb.2023.113569. Epub 2023 Sep 29.
4
Macrophages in the Inflammatory Phase following Myocardial Infarction: Role of Exogenous Ubiquitin.心肌梗死后炎症期的巨噬细胞：外源性泛素的作用
Biology (Basel). 2023 Sep 20;12(9):1258. doi: 10.3390/biology12091258.
5
hUC-MSC-EV-miR-24 enhances the protective effect of dexmedetomidine preconditioning against myocardial ischemia-reperfusion injury through the KEAP1/Nrf2/HO-1 signaling.人脐带来源间充质干细胞外泌体 miR-24 通过 KEAP1/Nrf2/HO-1 信号通路增强右美托咪定预处理对心肌缺血再灌注损伤的保护作用。
Drug Deliv Transl Res. 2024 Jan;14(1):143-157. doi: 10.1007/s13346-023-01388-7. Epub 2023 Aug 4.
6
Lyophilization Based Isolation of Exosomes.基于冷冻干燥的外泌体分离。
Int J Mol Sci. 2023 Jun 22;24(13):10477. doi: 10.3390/ijms241310477.
7
Extracellular lncRNAs secreted and absorbed by cardiomyocytes.心肌细胞分泌和吸收的细胞外 lncRNAs。
J Cell Biochem. 2023 Jun;124(6):785-796. doi: 10.1002/jcb.30425. Epub 2023 May 14.
8
Early growth response-1: Key mediators of cell death and novel targets for cardiovascular disease therapy.早期生长反应因子-1：细胞死亡的关键介质及心血管疾病治疗的新靶点
Front Cardiovasc Med. 2023 Mar 28;10:1162662. doi: 10.3389/fcvm.2023.1162662. eCollection 2023.
9
Plasma exosome miRNA-26b-3p derived from idiopathic short stature impairs longitudinal bone growth via the AKAP2/ERK1/2 axis.血浆外泌体 miRNA-26b-3p 来源于特发性身材矮小，通过 AKAP2/ERK1/2 轴损害纵向骨生长。
J Nanobiotechnology. 2023 Mar 16;21(1):94. doi: 10.1186/s12951-023-01849-8.
10
Microneedle Patch Loaded with Exosomes Containing MicroRNA-29b Prevents Cardiac Fibrosis after Myocardial Infarction.载有 microRNA-29b 的外泌体的微针贴片可预防心肌梗死后的心脏纤维化。
Adv Healthc Mater. 2023 May;12(13):e2202959. doi: 10.1002/adhm.202202959. Epub 2023 Feb 5.