Liu Ruina, Han Qi, Lin Geer, Mu Shuaicheng, Liu Shuang, Yao Su, Zhai Lei
China Center of Industrial Culture Collection (CICC), China National Research Institute of Food and Fermentation Industries, Beijing, 100015, People's Republic of China.
Curr Microbiol. 2025 Feb 13;82(4):135. doi: 10.1007/s00284-024-04056-2.
In recent years, as excellent industrial microorganisms, Halomonas has become a potential chassis cell of the next generation of industrial biotechnology because of its advantages of low complexity, antipollution ability, and rapid fermentation. Therefore, there is an urgent need to study the genome information, synthetic biology, multiomics, and other technologies of Halomonas, and it is also highly important to study its tolerance to extreme environments. Halomonas alkalicola CICC 11012 s is the most alkaliphilic bacterium in the genus Halomonas and is an excellent alkali-resistant bacterium that was independently isolated in our laboratory; this bacterium plays a certain role in industrial pollution control and the application of synthetic biology chassis cells. The H. alkalicola mutant was designed and constructed via CRISPR technology in the early stage of this experiment, which verified that the tonb gene plays an important role in the alkali resistance mechanism of this strain. Therefore, the molecular mechanism of the response of H. alkalicola CICC 11012 s to alkaline stress was explored through combined proteomic and metabolomic analysis. The experimental results revealed that the wild-type and mutant strains evolved multilevel adaptive strategies to regulate pH homeostasis in response to alkaline stress, including increasing their membrane transport activities and synthesizing carbohydrates and amino acids. In summary, the experimental results provide a deep understanding of the alkaline response mechanism of alkalophilic bacteria, thereby further promoting their application in different environments.
近年来，嗜盐单胞菌作为优良的工业微生物，因其具有基因组简单、抗污染能力强和发酵速度快等优点，已成为下一代工业生物技术潜在的底盘细胞。因此，迫切需要研究嗜盐单胞菌的基因组信息、合成生物学、多组学等技术，研究其对极端环境的耐受性也非常重要。嗜碱嗜盐单胞菌CICC 11012 s是嗜盐单胞菌属中最耐碱的细菌，是本实验室自主分离的优良耐碱菌；该菌在工业污染治理和合成生物学底盘细胞应用中发挥着一定作用。本实验前期通过CRISPR技术设计构建了嗜碱嗜盐单胞菌突变体，验证了tonb基因在该菌株耐碱机制中起重要作用。因此，通过蛋白质组学和代谢组学联合分析，探索了嗜碱嗜盐单胞菌CICC 11012 s对碱性胁迫响应的分子机制。实验结果表明，野生型和突变株在碱性胁迫下进化出多层次的适应性策略来调节pH稳态，包括增加膜转运活性以及合成碳水化合物和氨基酸。综上所述，实验结果为嗜碱细菌的碱性响应机制提供了深入了解，从而进一步促进其在不同环境中的应用。
