School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China.
School of Life Sciences, Tsinghua University, Beijing 100084, China.
Essays Biochem. 2021 Jul 26;65(2):393-403. doi: 10.1042/EBC20200159.
With the rapid development of systems and synthetic biology, the non-model bacteria, Halomonas spp., have been developed recently to become a cost-competitive platform for producing a variety of products including polyesters, chemicals and proteins owing to their contamination resistance and ability of high cell density growth at alkaline pH and high salt concentration. These salt-loving microbes can partially solve the challenges of current industrial biotechnology (CIB) which requires high energy-consuming sterilization to prevent contamination as CIB is based on traditional chassis, typically, Escherichia coli, Bacillus subtilis, Pseudomonas putida and Corynebacterium glutamicum. The advantages and current status of Halomonas spp. including their molecular biology and metabolic engineering approaches as well as their applications are reviewed here. Moreover, a systematic strain engineering streamline, including product-based host development, genetic parts mining, static and dynamic optimization of modularized pathways and bioprocess-inspired cell engineering are summarized. All of these developments result in the term called next-generation industrial biotechnology (NGIB). Increasing efforts are made to develop their versatile cell factories powered by synthetic biology to demonstrate a new biomanufacturing strategy under open and continuous processes with significant cost-reduction on process complexity, energy, substrates and fresh water consumption.
随着系统和合成生物学的快速发展，最近开发出了非模式细菌——盐单胞菌属（Halomonas spp.），由于其具有抗污染能力和在碱性 pH 值和高盐浓度下能够高密度生长的能力，成为了一种具有成本竞争力的平台，可以生产各种产品，包括聚酯、化学品和蛋白质。这些嗜盐微生物可以部分解决当前工业生物技术（CIB）的挑战，因为 CIB 基于传统底盘，通常是大肠杆菌（Escherichia coli）、枯草芽孢杆菌（Bacillus subtilis）、恶臭假单胞菌（Pseudomonas putida）和谷氨酸棒杆菌（Corynebacterium glutamicum），需要高能耗的灭菌来防止污染。本文综述了盐单胞菌属（Halomonas spp.）的优势和现状，包括其分子生物学和代谢工程方法及其应用。此外，还总结了一种系统的菌株工程流程，包括基于产品的宿主开发、遗传元件挖掘、模块化途径的静态和动态优化以及受生物过程启发的细胞工程。所有这些发展导致了下一代工业生物技术（NGIB）这一术语的出现。人们正在努力开发其多功能细胞工厂，为开放式连续工艺提供动力，在降低工艺复杂性、能源、底物和新鲜水消耗方面具有显著成本优势，展示了一种新的生物制造策略。
