Iksanova A M, Ojovan I M, Arzumanian V G, Vartanova N O, Poddubikov A V, Kolyganova T I, Konanykhina S Yu
I. Mechnikov Research Institute for Vaccines and Sera, Moscow, Russia.
I. M. Sechenov First Moscow State Medical University (Sechenov University, Ministry of Health of the Russian Federation, Moscow, Russia.
Bull Exp Biol Med. 2025 Jan;178(3):339-345. doi: 10.1007/s10517-025-06333-x. Epub 2025 Feb 13.
We studied the sensitivity of clinically significant (opportunistic) bacteria to blood serum fraction with a molecular weight <100 kDa containing antimicrobial peptides and proteins. The degree of damage to cytoplasmic membranes of bacterial cells was assessed by a spectrophotometric method. A total of 129 bacterial isolates belonging to 46 species, 19 genera, 13 families, 8 orders, 4 classes, and 3 types were studied. The mean sensitivity varied from -5.2 to 36.6%. The sensitivity depends on the structure of bacterial cell wall: higher sensitivity was demonstrated by taxonomic ranks related to gram-negative bacteria (median 19.1%) and lower values were observed in gram-positive bacteria (median 9.3%) (p<0.01). Among all orders, Lactobacilliales showed the lowest sensitivity (3.6%). Ranking of the studied species by their sensitivity showed that 7 of the first 15 species with sensitivity >19% are among to the leading causative agents of sepsis.
我们研究了具有临床意义的（机会性）细菌对分子量<100 kDa、含有抗菌肽和蛋白质的血清组分的敏感性。通过分光光度法评估细菌细胞质膜的损伤程度。共研究了属于46个物种、19个属、13个科、8个目、4个纲和3个类型的129株细菌分离株。平均敏感性在-5.2%至36.6%之间。敏感性取决于细菌细胞壁的结构：与革兰氏阴性菌相关的分类等级表现出较高的敏感性（中位数为19.1%），而革兰氏阳性菌的敏感性较低（中位数为9.3%）（p<0.01）。在所有目中，乳杆菌目表现出最低的敏感性（3.6%）。根据敏感性对所研究的物种进行排名显示，敏感性>19%的前15个物种中有7个是败血症的主要病原体。
