Yakovleva I V, Gavrilova N F, Kurbatova E A, Vorobyev D S, Petukhova E S, Semenova I B, Zaitsev A E, Volokh Yu V, Leonova A Yu, Poddubikov A V
I. Mechnikov Research Institute for Vaccines and Sera, Moscow, Russia.
Bull Exp Biol Med. 2025 Jan;178(3):346-350. doi: 10.1007/s10517-025-06334-w. Epub 2025 Feb 13.
Monoclonal antibodies (mAbs) of the IgG1 isotype obtained to the full-length recombinant pneumolysin (rPly) did not recognize or poorly recognized conformational epitopes of native pneumolysin in ELISA. At the same time, polyclonal antibodies (pAbs(rPly)) detected native pneumolysin in sandwich ELISA when they were used as capture and detecting antibodies simultaneously. All mAbs(rPly) inhibited erythrocyte hemolysis induced by native pneumolysin. The combined use of mAbs(rPly) did not reveal an increase in optical density in ELISA and an increase in inhibition hemolytic activity, which suggests that mAbs(rPly) are directed to the same or spatially closely located epitopes. pAbs(rPly) more effectively inhibited erythrocyte hemolysis than mAbs(rPly).
针对全长重组肺炎溶血素（rPly）获得的IgG1同种型单克隆抗体（mAbs）在酶联免疫吸附测定（ELISA）中无法识别或只能微弱识别天然肺炎溶血素的构象表位。与此同时，当多克隆抗体（pAbs(rPly)）同时用作捕获抗体和检测抗体时，其在夹心ELISA中能够检测到天然肺炎溶血素。所有mAbs(rPly)均能抑制天然肺炎溶血素诱导的红细胞溶血。mAbs(rPly)联合使用并未使ELISA中的光密度增加，也未使溶血抑制活性增强，这表明mAbs(rPly)针对的是相同或空间位置紧密相邻的表位。pAbs(rPly)比mAbs(rPly)更有效地抑制红细胞溶血。
