I. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia.
Bull Exp Biol Med. 2024 Jun;177(2):248-251. doi: 10.1007/s10517-024-06166-0. Epub 2024 Aug 1.
We compared the immunogenicity of recombinant S. pneumoniae pneumolysin (rPly) when administered with and without Al(OH) adjuvant, and evaluated the protective properties of recombinant protein in the active defense experiment. It was shown that double immunization with rPly+Al(OH) increases the levels of IgG antibodies in comparison with the control (p<0.01), while triple immunization results in a more significant increase in IgG antibody levels (p<0.001). Double immunization with rPly without Al(OH) does not induce a significant increase in antibody levels in comparison with the control, while triple immunization results in a slight but significant increase in antibody levels (p<0.05). The active defense test proved the protective activity of rPly against S. pneumoniae serotype 3 at intranasal infection.
我们比较了含有或不含有 Al(OH)佐剂的重组肺炎链球菌肺炎溶血素 (rPly) 的免疫原性，并在主动防御实验中评估了重组蛋白的保护特性。结果表明，与对照组相比，rPly+Al(OH) 双重免疫可显著增加 IgG 抗体水平（p<0.01），而三重免疫则可导致 IgG 抗体水平显著增加（p<0.001）。rPly 无 Al(OH) 双重免疫与对照组相比，抗体水平无显著增加，而三重免疫则可导致抗体水平略有但显著增加（p<0.05）。主动防御实验证明了 rPly 对鼻内感染的肺炎链球菌 3 型的保护活性。
