Shah Asjad Umair, Ali Khan Jawaria, Avais Muhammad, Zaman Syed Haider, Munir Zubir, Abbas Safdar, Tariq Mahnoor, Rahman Muneeb Ur, Tariq Fiza, Nawaz Saqib, Fouad Dalia, Anjum Aftab Ahmad, Ahmed Qazi Israr
Department of Veterinary Medicine, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Livestock and Dairy Development Department, Government of Punjab, Lahore, Pakistan.
PLoS One. 2025 Feb 13;20(2):e0315480. doi: 10.1371/journal.pone.0315480. eCollection 2025.
This study aimed to isolate and characterize Staphylococcus aureus (S. aureus) from samples of mastitis milk taken from dairy cattle in Punjab's Narowal District. 200 milk samples were collected aseptically from different dairy herds in the district, including clinical mastitis (CM) and sub-clinical mastitis (SCM) cows. Blood agar and mannitol salt agar were used for S. aureus isolation and identification. Selective media were then used for additional purification. Observations of morphological and biochemical traits verified the existence of S. aureus. Through questionnaire-based surveys, the prevalence of S. aureus mastitis was identified, and risk variables linked to its incidence were evaluated. The findings showed that S. aureus mastitis was prevalent in 42.5% of cases, with sub-clinical cases having a greater incidence (45.8%) than clinical cases (37.5%). Risk factors such as distance from dung pile to stall(m), source of water, dung removal per day, lactation period (weeks), parity, effect of milk yield (L), traumatic injury of udder, housing type, floor type, teat dipping, and bedding type was identified and their relationship to the occurrence of S. aureus mastitis was studied. Tests for antibiotic sensitivity revealed that S. aureus isolates were responsive to gentamycin, ceftiofur, tetracycline, enrofloxacin, and ciprofloxacin but extremely resistant to penicillin and amoxicillin. Additionally, the Somatic Cell Count (SCC) and California Mastitis Test (CMT) were used at different intervals to assess the effectiveness of the medication. Furthermore, compared to other treatment groups, a larger percentage of cure rates was seen in the groups receiving ceftiofur and enrofloxacin. Overall, this study contributes to the development of more effective management methods for S. aureus mastitis in dairy calves by offering insightful information about the condition's prevalence, risk factors, antibiotic sensitivity, and effectiveness of treatment.
本研究旨在从旁遮普省纳罗瓦尔区奶牛的乳腺炎乳汁样本中分离并鉴定金黄色葡萄球菌（S. aureus）。从该地区不同奶牛群中无菌采集了200份乳汁样本，包括临床型乳腺炎（CM）和亚临床型乳腺炎（SCM）奶牛的样本。使用血琼脂和甘露醇盐琼脂进行金黄色葡萄球菌的分离和鉴定。然后使用选择性培养基进行进一步纯化。通过对形态学和生化特征的观察证实了金黄色葡萄球菌的存在。通过问卷调查确定了金黄色葡萄球菌乳腺炎的患病率，并评估了与其发病相关的风险变量。结果表明，金黄色葡萄球菌乳腺炎在42.5%的病例中普遍存在，亚临床病例的发病率（45.8%）高于临床病例（37.5%）。确定了诸如粪堆到牛栏的距离（米）、水源、每日清粪量、泌乳期（周）、胎次、产奶量（升）的影响、乳房外伤、牛舍类型、地面类型、乳头药浴和垫料类型等风险因素，并研究了它们与金黄色葡萄球菌乳腺炎发生的关系。抗生素敏感性测试表明，金黄色葡萄球菌分离株对庆大霉素、头孢噻呋、四环素、恩诺沙星和环丙沙星有反应，但对青霉素和阿莫西林极具抗性。此外，在不同时间间隔使用体细胞计数（SCC）和加利福尼亚乳腺炎检测（CMT）来评估药物治疗的效果。此外，与其他治疗组相比，接受头孢噻呋和恩诺沙星治疗的组治愈率更高。总体而言，本研究通过提供有关该疾病的患病率、风险因素、抗生素敏感性和治疗效果的深刻信息，为制定更有效的奶牛金黄色葡萄球菌乳腺炎管理方法做出了贡献。
