一种用于体外测定人血清中卵磷脂 - 胆固醇酰基转移酶反应初始线性速率的简化方法。

A simplified procedure for the in vitro assay of the initial linear rate of the reaction of lecithin-cholesterol acyltransferase in human serum.

作者信息

Mahadevan V, Soloff L A

出版信息

Biochem Med. 1985 Feb;33(1):110-6. doi: 10.1016/0006-2944(85)90133-4.

Abstract

A simple sensitive method for the determination of the initial rate of the reaction of lecithin-cholesterol acyltransferase by equilibrating [3H]cholesterol with unesterified cholesterol of human serum is described. The resulting serum is incubated for various time periods at 37 degrees C and the increase of the label in the cholesterol ester fraction is measured. The labeling is effected by a filter paper method in which a paper strip containing the labeled cholesterol is placed in serum at 4 degrees C, thereby preventing the formation of labeled cholesterol esters by the action of the enzyme. The rate of the reaction was linear up to 30 min.

摘要

描述了一种通过使[3H]胆固醇与人血清中未酯化胆固醇达到平衡来测定卵磷脂 - 胆固醇酰基转移酶反应初始速率的简单灵敏方法。将所得血清在37℃下孵育不同时间段，然后测量胆固醇酯部分中标记物的增加量。标记通过滤纸法实现，即将含有标记胆固醇的纸条置于4℃的血清中，从而防止酶作用形成标记胆固醇酯。反应速率在30分钟内呈线性。