功能性光学相干断层扫描技术在植物声音相互作用研究中的应用
Functional optical coherence tomography in the study of plant-sound interactions.
作者信息
Rajagopalan Uma Maheswari, Wakumoto Ryotaro, Kono Takahiro, Kadono Hirofumi, Yamada Jun
机构信息
Faculty of Engineering, Shibaura Institute of Technology, 3-7-5 Toyosu, Koto-ku, Tokyo, 135-8548, Japan.
Tokyo Metropolitan University, Tokyo, Japan.
出版信息
BMC Res Notes. 2025 Feb 13;18(1):66. doi: 10.1186/s13104-025-07131-4.
OBJECTIVE
We aim to test the feasibility of applying Optical Coherence Tomography (OCT), a non-destructive, non-contact, real-time imaging technique to investigate the internal changes within leaves under sound stimulation.
RESULTS
Application of a spectral-domain OCT operating at 836.1 nm revealed that depth-resolved images obtained under no sound and under the application of sound stimuli of 100 Hz and 10 kHz sound at 100dB sound pressure to arugula (Eruca sativa) for a minute revealed a clear frequency dependence. The ratio of the OCT structural x-z images of a month-old arugula seedlings was obtained before and after the application of sound. From the ratio images, it was found that the effects under low and high-frequency sounds differed. The low-frequency sound of 100 Hz showed increased activity within the leaf compared to a high frequency of 10 kHz. Our results demonstrate that OCT can potentially investigate the effects of sound in plants speedily and thus has implications in agriculture applications.
目的
我们旨在测试应用光学相干断层扫描（OCT）的可行性，OCT是一种无损、非接触的实时成像技术，用于研究声音刺激下叶片内部的变化。
结果
使用工作在836.1nm的光谱域OCT，发现在无声音以及对芝麻菜（Eruca sativa）施加100dB声压的100Hz和10kHz声音刺激一分钟后获得的深度分辨图像显示出明显的频率依赖性。在施加声音之前和之后获取了一个月大的芝麻菜幼苗的OCT结构x-z图像的比率。从比率图像中发现，低频和高频声音下的效果不同。与10kHz的高频声音相比，100Hz的低频声音使叶片内的活性增加。我们的结果表明，OCT有可能快速研究声音对植物的影响，因此在农业应用中具有重要意义。
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