Maheswari R Uma, Takaoka H, Kadono H, Homma R, Tanifuji M
Laboratory for Integrative Neural Systems, Brain Science Institute, RIKEN, 2-1 Hirosawa, Wako, Saitama, Japan.
J Neurosci Methods. 2003 Mar 30;124(1):83-92. doi: 10.1016/s0165-0270(02)00370-9.
Mapping of the activity of brain by optical intrinsic signal imaging (OISI) provides a two-dimensional activation pattern of visual cortical areas at a resolution of a few hundred microns. However, integration of the intrinsic signal over depth results in loss of finer information about functional organization across the depth. Here, we report the first successful implementation of optical coherence tomography (OCT) at around 30 microm depth resolution to investigate cortical functions of a cat brain in vivo. This technique, named functional OCT (fOCT) provided visually evoked changes in the OCT signal. The fOCT signal shows stimulus specificity that correlates well with that of the intrinsic signals and provides depth resolved layer specific functional information.
通过光学内在信号成像（OISI）对大脑活动进行映射，可在几百微米的分辨率下提供视觉皮层区域的二维激活模式。然而，将内在信号在深度上进行整合会导致丢失有关跨深度功能组织的更精细信息。在此，我们报告了首次成功实施光学相干断层扫描（OCT），其深度分辨率约为30微米，用于在体研究猫脑的皮层功能。这项技术，称为功能性OCT（fOCT），提供了OCT信号中视觉诱发的变化。fOCT信号显示出刺激特异性，与内在信号的刺激特异性相关性良好，并提供深度分辨的层特异性功能信息。
