Liu Yi-Nan, Liu Zhourui, Liu Jian, Hu Yidan, Cao Bin
School of Civil and Environmental Engineering, Nanyang Technological University, 639798, Singapore; Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, 637551, Singapore.
Department of Biological Sciences and Technology, School of Environmental Studies, China University of Geosciences, Wuhan, 430074, China.
Metab Eng. 2025 May;89:1-11. doi: 10.1016/j.ymben.2025.02.002. Epub 2025 Feb 12.
Shewanella species are facultative anaerobes with distinctive electrochemical properties, making them valuable for applications in energy conversion and environmental bioremediation. Due to their well-characterized electron transfer mechanisms and ease of genetic manipulation, Shewanella spp. have emerged as a promising chassis for metabolic engineering. In this review, we provide a comprehensive overview of the advancements in Shewanella-based metabolic engineering. We begin by discussing the physiological characteristics of Shewanella, with a particular focus on its extracellular electron transfer (EET) capability. Next, we outline the use of Shewanella as a metabolic engineering chassis, presenting a general framework for strain construction based on the Design-Build-Test-Learn (DBTL) cycle and summarizing key advancements in the engineering of Shewanella's metabolic modules. Finally, we offer a perspective on the future development of Shewanella chassis, highlighting the need for deeper mechanistic insights, rational strain design, and interdisciplinary collaboration to drive further progress.
希瓦氏菌属是兼性厌氧菌，具有独特的电化学特性，这使其在能量转换和环境生物修复应用中具有重要价值。由于其电子传递机制已得到充分表征且易于进行基因操作，希瓦氏菌已成为代谢工程中一个有前景的底盘生物。在本综述中，我们全面概述了基于希瓦氏菌的代谢工程进展。我们首先讨论希瓦氏菌的生理特性，特别关注其细胞外电子传递（EET）能力。接下来，我们概述了将希瓦氏菌用作代谢工程底盘生物的情况，提出了基于设计-构建-测试-学习（DBTL）循环的菌株构建通用框架，并总结了希瓦氏菌代谢模块工程的关键进展。最后，我们对希瓦氏菌底盘生物的未来发展提出了展望，强调需要更深入的机制洞察、合理的菌株设计以及跨学科合作以推动进一步发展。
