Jones Meaghan J, Konwar Chaini, Asiimwe Rebecca, Dinh Louie, Razzaghian Hamid Reza, de Goede Olivia, MacIsaac Julia L, Morin Alexander M, Kolsun Kurt P, Gervin Kristina, Lyle Robert, Ng Raymond T, Koestler Devin C, Felix Janine F, Lavoie Pascal M, Robinson Wendy P, Mostafavi Sara, Kobor Michael S
Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, MB, Canada.
Children's Hospital Research Institute of Manitoba, Winnipeg, MB, Canada.
Commun Biol. 2025 Feb 14;8(1):237. doi: 10.1038/s42003-025-07661-4.
Epigenetic modifications such as DNA methylation are both cell type and developmental age specific. Here, we show that the immunological maturation of blood cell types influences DNA methylation changes from naive cord blood to fully functional adult blood. Lymphoid cells in adult blood showed more variability than in cord blood suggesting an antigen-dependent maturation of DNA methylation in lymphoid cells throughout the lifespan. Fewer DNA methylation changes between cord and adult blood were observed in myeloid cells, particularly in monocytes, which demonstrated the least number of DNA methylation changes between cord and adult blood. We also noted differences in epigenetic ages by immune cell types within the same individuals, specifically in cord blood where monocytes were epigenetically oldest compared to the other cell types. In addition, we provide a publicly available resource to the community as an R Shiny web application to interactively explore epigenetic patterns between naive cord white blood cells and fully functional adult white blood cells for six immune cell types.
诸如DNA甲基化之类的表观遗传修饰具有细胞类型和发育年龄特异性。在此，我们表明血细胞类型的免疫成熟会影响从原始脐带血到功能完全成熟的成人血液的DNA甲基化变化。成人血液中的淋巴细胞比脐带血中的淋巴细胞表现出更大的变异性，这表明在整个生命周期中，淋巴细胞中的DNA甲基化存在抗原依赖性成熟。在髓细胞中，尤其是单核细胞中，脐带血和成人血液之间观察到的DNA甲基化变化较少，单核细胞在脐带血和成人血液之间表现出最少的DNA甲基化变化。我们还注意到同一人体内免疫细胞类型的表观遗传年龄存在差异，特别是在脐带血中，与其他细胞类型相比，单核细胞的表观遗传年龄最大。此外，我们向社区提供了一个公开可用的资源，作为一个R Shiny网络应用程序，用于交互式探索六种免疫细胞类型的原始脐带白细胞和功能完全成熟的成人白细胞之间的表观遗传模式。
