Suzuki Takafumi, Tsujimoto Hironori, Watanabe Takanori, Ishibashi Yusuke, Fujishima Seiichiro, Itazaki Yujiro, Kariya Risa, Uehata Naoyuki, Shinada Hanae, Mochizuki Satsuki, Kishi Yoji, Sato Kimiya, Ueno Hideki
Department of Surgery, National Defense Medical College, Tokorozawa, Japan.
Department of Basic Pathology, National Defense Medical College, Tokorozawa, Japan.
Ann Surg Oncol. 2025 May;32(5):3839-3850. doi: 10.1245/s10434-025-16964-z. Epub 2025 Feb 14.
Neutrophil extracellular traps (NETs) have been implicated in cancer progression by enhancing cancer cell motility through the coiled-coil domain-containing protein 25 (CCDC25).
This study aimed to evaluate the prognostic value of CCDC25 expression in patients with esophageal squamous cell carcinoma (ESCC).
Tissue specimens from patients who underwent radical esophagectomy for ESCC were analyzed to investigate the relationship between CCDC25 expression, clinicopathological features, and prognosis. Western blotting was conducted to compare CCDC25 expression in tumor and non-tumorous tissues (n = 4). Immunohistochemical staining was performed to categorize patients into two groups based on CCDC25 expression: CCDC25 (n = 80) and CCDC25 (n = 40).
CCDC25 expression was significantly higher in tumor tissues compared with non-tumorous tissues (p = 0.006). Patients in the CCDC25 group had a higher likelihood of poorly differentiated tumors; however, no significant differences were observed in the other clinicopathological features between the two groups. High CCDC25 expression was associated with significantly lower relapse-free survival (RFS) rate (p = 0.03) but not overall survival (OS) [p = 0.07]. Multivariate analysis revealed that high CCDC25 expression and tumor depth were independent predictors of both RFS and OS. Furthermore, high CCDC25 expression was significantly associated with worse prognosis in patients with high postoperative neutrophil counts (≥ 9600/µL) and those with postoperative infectious complications.
High expression of CCDC25 was identified as an unfavorable prognostic factor in patients with ESCC, particularly in those with elevated postoperative neutrophil counts. Targeting CCDC25 could potentially improve prognosis in specific subgroups of patients with ESCC.
中性粒细胞胞外诱捕网（NETs）通过含卷曲螺旋结构域蛋白25（CCDC25）增强癌细胞迁移能力，从而与癌症进展有关。
本研究旨在评估CCDC25表达在食管鳞状细胞癌（ESCC）患者中的预后价值。
对接受ESCC根治性食管切除术患者的组织标本进行分析，以研究CCDC25表达、临床病理特征和预后之间的关系。进行蛋白质免疫印迹法比较肿瘤组织和非肿瘤组织中CCDC25的表达（n = 4）。进行免疫组织化学染色，根据CCDC25表达将患者分为两组：CCDC25高表达组（n = 80）和CCDC25低表达组（n = 40）。
与非肿瘤组织相比，肿瘤组织中CCDC25表达显著更高（p = 0.006）。CCDC25高表达组患者肿瘤低分化的可能性更高；然而，两组之间的其他临床病理特征未观察到显著差异。CCDC25高表达与无复发生存率（RFS）显著降低相关（p = 0.03），但与总生存率（OS）无关[p = 0.07]。多因素分析显示，CCDC25高表达和肿瘤深度是RFS和OS的独立预测因素。此外，CCDC25高表达与术后中性粒细胞计数高（≥ 9600/µL）的患者以及有术后感染并发症的患者预后更差显著相关。
CCDC25高表达被确定为ESCC患者的不良预后因素，尤其是术后中性粒细胞计数升高的患者。靶向CCDC25可能会改善ESCC特定亚组患者的预后。
